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The brush cells of the common bile duct of the rat

Thin section, freeze-fracture and scanning electron microscopy

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Summary

Two different fixative procedures (immersion and perfusion) and four different fixative solutions were used in order to obtain the best preservation of the brush cells of the common bile duct of the rat. The results indicate that only perfusion fixation through the common bile duct is suitable, independent of the fixative solutions and their osmolarity.

Numerous brush cells were seen in the proximal and distal regions of the common bile duct. In these locations, they could be implicated in a registration and/or regulation of intraluminal pressure variations.

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References

  • Atema J (1973) Microtubule theory of sensory transduction. J Theor Biol 38:181–190

    Google Scholar 

  • Atema J (1975) Stimulus transmission along microtubules in sensory cells: an hypothesis. In: M Borgers, M de Brabander (eds) “Microtubules and Microtubule Inhibitors”. Amsterdam North-Holland Publishing Company, p 247–257

    Google Scholar 

  • Bennett HS, Luft JH (1959) S-collidine as a basis for buffering fixatives. J Biophys Biochem Cytol 6:113–114

    Google Scholar 

  • Dowell WCT (1964) Die Entwicklung geeigneter Folien für elektronenmikroskopische Präparatträger großen Durchlaßbereiches und ihre Verwendung zur Untersuchung von Kristallen. Optik 21:47–58

    Google Scholar 

  • Dustin P (1978) Microtubules. Springer-Verlag, Berlin Heidelberg New York

    Google Scholar 

  • Fawcett DW, Heidger PM, Leak LV (1969) Lymph vascular system of the interstitial tissue of the testis as revealed by electron microscopy. J Reprod Fert 19:109–119

    Google Scholar 

  • Fox H, Whitear M (1978) Observations on Merkel cells in amphibians. Biol Cellulaire 32:223–232

    Google Scholar 

  • Franke WW, Schmidt E, Kartenbeck J, Mayer D, Hacker H-J, Bannasch P, Osborn M, Weber K, Denk H, Wanson J-C, Drochmans P (1979) Characterization of the intermediate-sized filaments in liver cells by immunofluorescence and electron microscopy. Biol Cellulaire 34:99–110

    Google Scholar 

  • Hallenbeck GA (1967) Biliary and pancreatic intraductal pressures. In: Handbook of Physiology, vol. II, chap. 57. American Physiol Soc, Washington, DC p 1007–1025

    Google Scholar 

  • Higgins GM (1926) The biliary tract of certain rodents with and those without a gall bladder. Anat Rec 32:89–111

    Google Scholar 

  • Isomäki AM (1973) A new cell type (tuft cell) in the gastro-intestinal mucosa of the rat. A transmission and scanning electron microscopic study. Acta Pathol Microbiol Scand Section A, Suppl 240:1–35

    Google Scholar 

  • Iurato S, Franke K, Luciano L, Wermbter G, Pannese E, Reale E (1976) Fracture faces of the junctional complexes in the reticular membrane of the organ of Corti. Acta Otolaryngol 81:36–47

    Google Scholar 

  • Leeson TS, Melax H (1969) Fine structure of the common bile duct in adult rats. Can J Zool 47:33–35

    Google Scholar 

  • Luciano L (1972) Die Feinstruktur der Gallenblase und der Gallengänge. II. Das Epithel der extrahepatischen Gallengänge der Maus und der Ratte. Z Zellforsch 135:103–114

    Google Scholar 

  • Luciano L, Reale E (1969) A new cell type (“brush cell”) in the gall bladder epithelium of the mouse. J Submicr Cytol 1:43–52

    Google Scholar 

  • Luciano L, Reale E (1979) A new morphological aspect of the brush cells of the mouse gallbladder epithelium. Cell Tissue Res 201:37–44

    Google Scholar 

  • Luciano L, Reale E, Ruska H (1968) Über eine “chemorezeptive” Sinneszelle in der Trachea. Z Zellforsch 85:350–375

    Google Scholar 

  • Luciano L, Castellucci M, Reale E (1977a) Die Feinstruktur der Gallenblase und der Gallengänge. VI. Rasterelektronenmikroskopische Beobachtungen an den Bürstenzellen des Choledochus der Ratte. Beitr elektronenmikroskop Direktabb Oberfl 10:469–476

    Google Scholar 

  • Luciano L, Franke K, Iurato S, Reale E (1977b) Freeze-fracture study of the cell junctions in the utricle and saccule. Acta Otolaryngol 83:79–84

    Google Scholar 

  • Luciano L, Thiele J, Reale E (1979) Development of follicles and of occluding junctions between the follicular cells of the thyroid gland. A thin-section and freeze-fracture study in the fetal rat. J Ultrastruct Res 66:164–181

    Google Scholar 

  • Luftig RB, McMillan PN, Weatherbee JA, Weihing RR (1977) Increased visualization of microtubules by an improved fixation procedure. J Histochem Cytochem 25:175–187

    Google Scholar 

  • Mann FC (1919) A study of the tonicity of the sphincter at the duodenal end of the common bile duct. J Lab Clinical Med 5:107–110

    Google Scholar 

  • Mann FC (1920) A comparative study of the anatomy of the sphincter at the duodenal end of the common bile-duct with special reference to species of animals without a gall-bladder. Anat Rec 18:355–360

    Google Scholar 

  • Mann FC, Brimhall SD, Foster JP (1920) The extrahepatic biliary tract in common domestic and laboratory animals. Anat Rec 18:47–66

    Google Scholar 

  • Matsumoto G, Sakai H (1979) Microtubules inside the plasma membrane of squid giant axons and their possible physiological functions. J Membrane Biol 50:1–14

    Google Scholar 

  • McMaster PD (1922) Do species lacking a gall bladder possess its functional equivalent? J Exp Med 35:127–140

    Google Scholar 

  • Millonig G (1961) Advantages of a phosphate buffer for OsO4 solutions in fixation. J Appl Phys 32:1637

    Google Scholar 

  • Moor H, Mühlethaler K (1963) Fine structure in frozen-etched yeast cells. J Cell Biol 17:609–628

    Google Scholar 

  • Nabeyama A, Leblond CP (1974) “Caveolated cells” characterized by deep surface invaginations and abundant filaments in mouse gastro-intestinal epithelia. Am J Anat 140:147–166

    Google Scholar 

  • Riches DJ (1972) Ultrastructural observations on the common bile duct epithelium of the rat. J Anat 111:157–170

    Google Scholar 

  • Ruska C (1961) Die Zellstruktur des Dünndarmepithels in ihrer Abhängigkeit von der physikalisch-chemischen Beschaffenheit des Darminhalts. II. Wasserlösliche, grenzflächen-aktive Stoffe. Z Zellforsch 53:867–878

    Google Scholar 

  • Schmidt CR, Ivy AC (1937) The general function of the gallbladder. J Cell Comp Physiol 10:365–383

    Google Scholar 

  • Simson JAV, Spicer SS (1975) Selective subcellular localization of cations with variants of the potassium (pyro)antimonate technique. J Histochem Cytochem 23:575–598

    Google Scholar 

  • Szamier P, Pollard T, Fugiwara K (1975) Tropomyosin prevents the destruction of actin filaments by osmium. J Cell Biol 67:424a

    Google Scholar 

  • Temmink JHM, Spiele H (1978) Preservation of cytoskeletal elements for electron microscopy. Cell Biol Internat Reports 2:51–59

    Google Scholar 

  • Yamada K (1969) Fine structure of rodent common bile duct epithelium. J Anat 105:511–523

    Google Scholar 

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Luciano, L., Castellucci, M. & Reale, E. The brush cells of the common bile duct of the rat. Cell Tissue Res. 218, 403–420 (1981). https://doi.org/10.1007/BF00210353

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