Summary
Bloodstains were made with 200 μl blood on each of 11 different common substrates to examine the effect of the stain carrier on the amount and quality of DNA recoverable. High-molecular-weight DNA was extracted from all samples after 2 days. The yield of DNA from each sample varied considerably, not only between the different stain carriers but also within a given category. With a DNA yield of up to 10 μg, paper, glass, nylon, wood, smooth leather and wool gave the best results, followed by blue denim and wallpaper (up to 6 μg), cotton fabric and carpeting (up to 4 μg), and suede (up to 2 μg), For several stain carriers the DNA-containing solution was contaminated by chemical substances, which in the case of the blue denim, suede, and carpet samples inhibited the digestion of the DNA with restriction enzymes and prevented DNA typing. The different textures of the stain carriers tested and (as for varying yields on the same carrier) the differing degree of loss of DNA during extraction and the physiological variation in the number of leukocytes in human blood are discussed as possible reasons for the wide range of variation in the amounts of DNA it was possible to extract.
Zusammenfassung
Auf elf verschiedenen, häufig vorkommenden Materialien wurden aus 200 μl Frischblut Spuren angelegt, um den Einfluß des Spurenträgers auf Menge and Qualität extrahierbarer DNA festzustellen. Nach der Lagerzeit von zwei Tagen konnte aus alien Proben hochmolekulare DNA gewonnen werden. Die jeweils isolierten Mengen schwankten sowohl innerhalb einer Kategorie, als auch zwischen den verschiedenen Spurenträgern erheblich. Mit bis zu 10 μg extrahierbarer DNA zeigten Papier, Glas, Nylon, Holz, glattes Leder and Wolle die besten Resultate, gefolgt von Bluejeansgewebe und Tapete (bis zu 6 μg), Baumwollstoff and Teppichboden (bis zu 4 μg) und Wildleder (bis zu 2 μg), Die erhaltene DNA-Lösung war in einigen Fällen durch aus dem Träger stammende chemische Substanzen verunreinigt, wodurch für die Spurenträger Bluejeansgewebe, Wildleder und Teppichboden das Schneiden der DNA mit Restriktionsenzymen und somit die Typisierung verhindert wurden. Als Ursache für die unterschiedlichen DNA-Ausbeuten werden die Trägereigenschaften und — wie auch für unterschiedliche Ergebnisse für den gleichen Träger —, der differierende Verlust bei den verschiedenen Extraktionsschritten and die physiologische Variation der Leukozytenanzahl der Spenderblute diskutiert.
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Dedicated to Professor Adebahr on the occasion of his 65th birthday
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Prinz, M., Berghaus, G. The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains. Z Rechtsmed 103, 191–197 (1990). https://doi.org/10.1007/BF00207340
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DOI: https://doi.org/10.1007/BF00207340