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Irradiated tumor cells of lipopolysaccharide stimulation elicit an enhanced anti-tumor immunity

  • Original Article – Cancer Research
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Purpose

Lipopolysaccharide (LPS) is a major component of the outer surface membrane of Gram-negative bacteria which has been proved an effective immune enhancer. Here, we investigated the anti-tumor effect of irradiated tumor cells that stimulated by LPS in mouse xenografts models.

Methods

Tumor cells were irradiated after stimulation with 1 μg/mL LPS for 48 h. The C57BL/6 mice were immunized subcutaneously with irradiated tumor cells. The anti-tumor effect of lymphocytes of immunized mice was investigated. The cytotoxicity of spleen lymphocytes from immunized mice was determined by a standard 51Cr-release assay. The roles of immune cell subsets in anti-tumor activity were assessed by injected intraperitoneally with monoclonal antibodies.

Results

We observed that the vaccine of irradiated tumor cell with LPS-stimulated elicited a stronger protective anti-tumor immunity than other controls. Adoptive transfer of lymphocytes of immunized mice showed that the cellular immune response was involved in the anti-tumor effect. And this effect was achieved by activation of antigen-specific CD8+ T cell response and reduction of myeloid-derived suppressor cells (MDSCs, Gr1+ CD11b+), which were confirmed by depletion of immune cell subsets and flow cytometry analysis.

Conclusions

In summary, our study showed that stimulation of LPS was able to enhance anti-tumor immunity of vaccination with tumor cells after irradiation treatment, which might be a new strategy for cancer therapy.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grants 81123003 and 81301980) and China Postdoctoral Science Foundation (2013M542283).

Conflict of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Gaopeng Street, Keyuan Road 4, Chengdu, 610041, Sichuan, People’s Republic of China

    Yuli Li, Guobo Shen, Zhimian Li, Yaxiong Sang & Yuquan Wei

  2. State Key Laboratory of Biotherapy, The Department of Medical Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Wen Nie & Binglan Zhang

Authors
  1. Yuli Li
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  2. Guobo Shen
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  3. Wen Nie
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  4. Zhimian Li
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  5. Yaxiong Sang
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  6. Binglan Zhang
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  7. Yuquan Wei
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Correspondence to Guobo Shen.

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Li, Y., Shen, G., Nie, W. et al. Irradiated tumor cells of lipopolysaccharide stimulation elicit an enhanced anti-tumor immunity. J Cancer Res Clin Oncol 140, 1815–1823 (2014). https://doi.org/10.1007/s00432-014-1721-4

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  • DOI: https://doi.org/10.1007/s00432-014-1721-4

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