Abstract
Fumonisin B1 and AAL-toxin, both of which contain sphingolipid-like substituents, are water-soluble metabolites of Fusarium moniliforme and Alternaria alternata, respectively. These two toxins were compared to each other and to tetraacetylphtosphingosine (TAPS) and triacetyldihydrosphingosine (TADS) for effects on chlorophyll production and growth in Lemna minor L. (duckweed). Fumonisin B1 (0.7 μg), TAPS, and TADS all produced parallel effects on growth rate and chlorophyll content; however, FB1 did so at a 33-fold lower concentration. The AAL-toxin at 0.7 μg affects chlorophyll content more than plant growth (36% versus 73%, respectively), whereas at 3.3 μg concentration, the growth rate was less than 50% and chlorophyll content was reduced by 80%. By contrast, the hydrolysis product of FB1 that does not contain the tricarballylic acid (TCA) substituent is 23 times less active, which suggests that this component somehow enhances activity. The yeast sphingolipids are completely acetylated and do not contain TCA groups but also affect chlorophyll content and growth rate of duckweed. However the effect was substantially less than with AAL-toxin and FB1, which contain one and two TCA groups, respectively.
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References
Abbas HK, Boyette CD, Hoagland RE, Vesonder RF (1991) Bioherbicidal potential of Fusarium moniliforme and its phytotoxin, fumonisin. Weed Sci 39:673–677
Bezuidenhout SC, Gelderblom WCA, Gorst-Allman CP, Horak RM, Marasas WFO, Spiteller G and Vleggar R (1988) Structure elucidation of the fumonisins, mycotoxins from Fusarium moniliforme. J Chem Soc Chem Commun:743–745
Bottini AY, Bowen JR, Gilchrist DG (1981) Phytotoxins II. Characterization of a phyto-toxic fraction from Alternaria alternata f sp lycopersici. Tetrahedron Lett 22:2723–2726
Einhellig FA, Leather GR, Hobbs LL (1985) Use of Lemna minor L. as a bioassay in allelopathy. J Chem Ecol 11:65–72
Fuson GB, Pratt D (1988) Effects of the host-specific toxins of Alterneria alternata f. sp. lycopersici on suspension-cultured tomato cells. Phytopathology 78:1641–1648
Gilchrist DG, Grogan RG (1976) Production and nature of host-specific toxin from Alternaria alternata f sp lycopersici. Phytopathology 66:165–171
Hannun YA, Bell RM (1989) Functions of sphingolipids and sphingolipid breakdown products in cellular regulation. Science 243:500–507
Kaneshiro T, Vesonder RF, Peterson RE (1992) Fumonisin-stimulated N-acetyldihydrosphingosine, N-acetylphytosphingosine, and phytosphingosine products of Pichia (Hansenula) ciferri, NRROL Y-1031. Current Microbiol 24:319–324
Marasas WFO, Kellerman TS, Gelderblom WCA, Coetzer JAW, Thiel PG, van der Lugt JJ (1988) Leukoencephalomalacia in a horse induced by fumonisin B1 isolated from Fusarium moniliforme Sheldon. Ondersterpoort J Vet Res 55:197–203
Mirocha CJ, Gilchrist DG, Martensen A, Abbas HK, Plasencia J, Vesonder RF (1990) Production of toxins with equivalent host-specific activity, fumonisin and AAL, by nonpathogens of tomato. Phytopathology (Abstract). Paper A370, APS/CPS Annual Meeting, Grand Rapids, MI. The American Phytopathological Society, St. Paul, MN
Morell P, Braun P (1972) Biosynthesis and metabolic degradation of sphingolipids not containing sialic acid. J Lipid Res 13:293–310
Shier WT, Abbas HK, Mirocha CJ (1991) Toxicity of the mycotoxins fumonisins B1 and B2 and Alternaria alternata f. sp. lycopersici toxin (AAL) in cultured mammalian cells. Mycopathologia 116:97–104
Stodola FH, Wickerham LJ, Scholfield CR, Dutton HJ (1962) Formation of extracellular sphingolipids by microorganisms. III Triacetyl dihydrosphingosine, a metabolic product of the yeast Hansenula ciferri. Arch Biochem Biophys 98:176
Vesonder RF, Labeda DP, Peterson RE (1992) Phytotoxic Activity of Selected Water-Soluble Metabolites of Fusarium on Lemna minor L. (Duckweed). Mycopathologia (in press)
Vesonder RF, Peterson RE, Plattner R, Weisleder D (1990) Fumonisin B1 Isolation from corn culture, and purification by high performance liquid chromatography. Mycotox Res 6:85–88
Wang E, Norred WP, Bacon CW, Riley RT, Merrill JR AH (1991) Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme. J Biol Chem. 266:14486–14490
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Vesonder, R.F., Peterson, R.E., Labeda, D. et al. Comparative phytotoxicity of the fumonisins, AAL-toxin and yeast sphingolipids in Lemna minor L. (duckweed). Arch. Environ. Contam. Toxicol. 23, 464–467 (1992). https://doi.org/10.1007/BF00203810
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DOI: https://doi.org/10.1007/BF00203810