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Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS

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Summary

A new human neuroblastoma cell line (LS) that originated from an abdominal tumor of a 16-month-old girl is presented; it was classified, according to Evans, as being stage III. Morphological (dense-core particles) and biochemical characteristics (dopamine-β-hydroxylase, acetylcholinesterase, neuron-specific-enolase) confirmed the diagnosis. In addition to a slightly variable modal chromosome number of 48 or 49 (because of marker-chromosomes and autosomal trisomies), cytogenetic analysis revealed two constantly appearing chromosomes with homogeneously stained regions (HSR's). The karyo-type remained constant over 50 passages in vitro [49,XX, −12,+der5, + 17,+mar1,+mar2]. Double minutes were a rare phenomenon and appeared only in a few metaphases. In situ hybridization showed that some of the HSR's consisted of amplified N-myc copies. The distribution of the N-myc copies according to in situ hybridization signals along the HSR's was compared with the data of Southern and Northern blotting analyses.

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Authors and Affiliations

  1. Abteilung Klinische Genetik, Institut für Anthropologie und Humangenetik der Universität, Wilhelmstrasse 27, W-7400, Tübingen, Federal Republic of Germany

    Günter Rudolph & Peter Kaiser

  2. Universitäts-Kinderklinik der Universität, Rümelinstrasse 19-23, W-7400, Tübingen, Federal Republic of Germany

    Karin Schilbach-Stückle & Rupert Handgretinger

  3. Abteilung Klinische Genetik der Universität, Frauenstrasse 29, W-7900, Ulm, Federal Republic of Germany

    Horst Hameister

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  1. Günter Rudolph
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  2. Karin Schilbach-Stückle
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  3. Rupert Handgretinger
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  4. Peter Kaiser
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  5. Horst Hameister
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Rudolph, G., Schilbach-Stückle, K., Handgretinger, R. et al. Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS. Hum Genet 86, 562–566 (1991). https://doi.org/10.1007/BF00201542

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  • DOI: https://doi.org/10.1007/BF00201542

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