Abstract
The lateral leaflets of Desmodium motorium exhibit rhythmic upward and downward movements with a period in the minute range. Apoplasmic K+ and H+ activities were monitored in situ in the abaxial part of the pulvini with ion-selective microelectrodes. An extracellular electric potential was recorded simultaneously. The apoplasmic H+ activity of all pulvini exhibiting a regular rhythm of the extracellular electric potential oscillated with the same period between about 10 and 20 mM. The apoplasmic K+ activity was high when the membrane potential of the motor cells was depolarized (about 36 mV) and the cells were shrunken. In contrast, the apoplasmic K+ activity was low in the swollen state of the motor cells, when the membrane potential was hyperpolarized (about -136 mV). The volatile anesthetic enflurane suppressed reversibly the movement of the leaflets. The same treatment also arrested spontaneous oscillations in the apoplasmic K+ activity in the pulvinus. The apoplasmic K+ activity oscillated roughly in phase with the K+ activity between pH 6.6 and 6.0. Application of white light disturbed the rhythm and increased the extracellular pH. Our results indicate that the physiological mechanism that drives the lateral leaflet movements of Desmodium motorium is closely related to the osmotic motors mediating the leaf movements of Mimosa, Samanea and Phaseolus.
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Abbreviations
- Em :
-
membrane potential
- Eex :
-
extracellular electric potential
- Hex :
-
extracellular H+ activity
- Kex :
-
extracellular K+ activity
- Rex :
-
extracellular electrical resistance
References
Abe, T., Oda, K. (1976) Resting and action potentials of excitable cells in the main pulvinus of Mimosa pudica. Plant Cell Physiol. 17, 1343–1346
Antkowiak, B (1992) Elektrophysiologische Untersuchungen zur Seitenfiederbewegung von Desmodium motorium. Ph. D.-Thesis, University of Tübingen
Antkowiak, B., Engelmann, W. (1989) Ultradian rhythms in the pulvini of Desmodium motorium — an electrophysiological approach. J. Interdiscip. Cycle Res. 20, 164–165
Antkowiak, B., Kirschfeld, K. (1992) Enflurane is a potent inhibitor of high conductance Ca2+-activated K+ channels of Chara australis. FEBS Lett. 313, 218–221
Antkowiak, B., Mayer, W.-E., Engelmann, W. (1991) Oscillations of the membrane potential of pulvinar motor cells in situ in relation to leaflet movements of Desmodium motorium. J. Exp. Bot. 42, 901–910
Antkowiak, B., Engelmann, W., Herbjørnsen, R., Johnsson, A. (1992) Effects of vanadate, N2 and light on the membrane potential of motor cells and the lateral leaflet movements of Desmodium motorium. Physiol. Plant. 86, 551–558
Bose, J.C. (1913) Research on irritability of plants. Longmans, Green and Co., London
Freudling, C., Mayer, W.-E., Gradmann, D. (1980) Electrical membrane properties and circadian rhythm in extensor cells of the laminar pulvini of Phaseolus coccineus L. Plant Physiol. 65, 966–968
Freudling, C., Starrach, N., Flach, D., Gradmann, D., Mayer, W.E. (1988) Cell walls as reservoirs of potassium ions for reversible volume changes of pulvinar motor cells during rhythmic leaf movements. Planta 175, 193–203
Fromm, J., Eschrich, W. (1990) Seismonastic movements in Mimosa. In: The pulvinus: Motor organ of leaf movement, pp. 25–43, Satter, R.L., Gorton, H.L., Vogelmann, T.C., eds. Am. Soc. Plant Physiol., Rockville, Md.
Guhathakurta, A., Dutt, B.K. (1961) Electrical correlate of the rhythmic pulsatory movement of Desmodium gyrans. Transact. Bose Res. Inst. 24, 73–82
Johnsson, A., Engelmann, W., Antkowiak, B. (1990) Leaf movements in nyctinastic plants as hands of the biological clock. In: The pulvinus: Motor organ of leaf movement, pp. 79–101, Satter, R.L., Gorton, H.L., Vogelmann, T.C., eds. Am. Soc. Plant Physiol., Rockville, Md.
Kumon, K., Suda, S. (1984) Ionic fluxes from pulvinar cells during the rapid movement of Mimosa pudica. L. Plant Cell Physiol. 23, 975–979
Kumon, K., Suda, S. (1985) Changes in extracellular pH of the motor cells of Mimosa pudica L. during movement. Plant Cell Physiol. 26, 375–377
Kumon, K., Tsurumi, S. (1984) Ion efflux from pulvinar cells during slow downward movement of the petiole of Mimosa pudica L. induced by photostimulation. J. Plant Physiol. 115, 439–443
Lee, Y. (1990) Ion movements that control pulvinar curvature in nyctinastic legumes. In: The pulvinus: Motor organ of leaf movement, pp. 130–141, Satter, R.L., Gorton, H.L., Vogelmann, T.C., eds. Am. Soc. Plant Physiol., Rockville, Md.
Lee, Y., Satter, R.L. (1989) Effects of white, blue, red light and darkness on pH of the apoplast in the Samanea pulvinus. Planta 178, 31–40
Mayer, W.-E. (1990) Walls as potassium storage reservoirs in Phaseolus pulvini. In: The pulvinus: Motor organ of leaf movement, pp. 160–174, Satter, R.L., Gorton, H.L., Vogelmann, T.C., eds. Am. Soc. Plant Physiol., Rockville, Md.
Mayer, W.-E., Flach, D., Raju, M.V.S., Starrach, N., Wiech, E. (1985) Mechanics of circadian pulvini movements in Phaseolus coccineus L. Planta 163, 381–390
Mitsuno, T., Sibaoka, T. (1989) Rhythmic electrical potential change of motor pulvinus in lateral leaflet of Codariocalyx motorius. Plant Cell Physiol. 30, 1123–1127
Moran, N. (1990) The role of ion channels in osmotic volume changes in Samanea motor cells analysed by patch-clamp methods, pp. 142–159. In: The pulvinus: Motor organ of leaf movement, Satter, R.L., Gorton, H.L., Vogelmann, T.C., eds. Am. Soc. Plant Physiol., Rockville, Md.
Nishizaki, Y. (1987) Light-induced changes of electrical potential in pulvinar motor cells of Phaseolus vulgaris L. Plant Cell Physiol. 28, 1163–1166
Nishizaki, Y. (1988) Blue light pulse-induced transient changes of electric potential and turgor pressure in the motor cells of Phaseolus vulgaris L. Plant Cell Physiol. 29, 1041–1046
Samejima, M., Sibaoka, T. (1980) Changes in the extracellular ion concentration in the main pulvinus of Mimosa pudica during rapid movement and recovery. Plant Cell Physiol. 21, 467–479
Sanders, D. (1980) The mechanism of Cl− transport at the plasma membrane of Chara corrallina: I. Cotransport with H+. J. Membr. Biol. 53, 129–141
Sanders, D., Hansen, U.-P. (1981) Mechanism of Cl− transport at the plasma membrane of Chara corralina: II. Transinhibition and the determination of H+/C1− binding order from a reaction kinetic model. J. Membr. Biol. 58, 139–153
Satter, R.L., Morse, M.J., Lee, Y., Crain, R.C., Coté, G.G., Moran, N. (1988) Light- and clock-controlled leaflet movements in Samanea saman: A physiological, biophysical and biochemical analysis. Bot. Acta 101, 205–213
Schuster, J., Engelmann, W. (1990) Recording of rhythms in organisms using video-digitizing. In: Chronobiology: Its role in clinical medicine, general biology and agriculture, pp. 389–396, Hayes, D.K., Pauly, J.E., Reiter, R.J., eds. WileyLiss, New York
Starrach, N., Mayer, W.-E. (1989) Changes of the apoplasmic pH and K+ concentration in Phaseolus pulvinus in situ in relation to rhythmic leaf movements. J. Exp. Bot 40, 865–873
Starrach, N., Freudling, C., Mayer, W.-E., Gradmann, D. (1984) Aged preparations are electrophysiologically more representative of intact tissue than are freshly excised preparations. Planta 160, 88–90
Tazawa, M., Shimmen, T., Mimura, T. (1987) Membrane control in the Characeae. Annu. Rev. Plant Physiol. 38, 95–117
Zucker Lowen, C., Satter, R.L. (1989) Light-promoted changes in apoplastic K+ activity in the Samanea saman pulvinus, monitored with liquid membrane microelectrodes. Planta 179, 421–427
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B. Antkowiak was supported by the Stiftung Volkswagenwerk.
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Antkowiak, B., Engelmann, W. Oscillations of apoplasmic K+ and H+ activities in Desmodium motorium (Houtt.) Merril. pulvini in relation to the membrane potential of motor cells and leaflet movements. Planta 196, 350–356 (1995). https://doi.org/10.1007/BF00201395
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DOI: https://doi.org/10.1007/BF00201395