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Granulocyte-monocyte-colony-stimulating factor augments the cytotoxic capacity of lymphocytes and monocytes in antibody-dependent cellular cytotoxicity

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Summary

Human peripheral blood mononuclear cells (lymphocytes and monocytes) were preincubated for 0–24 h with human recombinant granulocyte-monocyte-colony-stimulating factor (GM-CSF) and used as effector cells in an 18 h antibody-dependent cellular cytotoxicity (ADCC) assay with SW948 (a human colorectal carcinoma cell line) as target cells and mAb 17-1A. A significant increase in the lytic capability was noted after 0.5–2 h of preactivation while longer preincubation times did not significantly increase the lytic potential. GM-CSF at 0.01 μg/ml induced the best tumor cell lysis while higher concentrations were inhibitory. GM-CSF pretreatment induced a statistically significant increase in the lytic capacity of both monocytes and lymphocytes in ADCC as well as in the spontaneous cytotoxicity.

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Authors and Affiliations

  1. Department of Oncology (Radiumhemmet) and Immunologic Research Laboratory, Karolinska Hospital, S-104 01, Stockholm

    G. Masucci, P. Wersäll, P. Ragnhammar & H. Mellstedt

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  1. G. Masucci
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  2. P. Wersäll
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  3. P. Ragnhammar
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  4. H. Mellstedt
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Masucci, G., Wersäll, P., Ragnhammar, P. et al. Granulocyte-monocyte-colony-stimulating factor augments the cytotoxic capacity of lymphocytes and monocytes in antibody-dependent cellular cytotoxicity. Cancer Immunol Immunother 29, 288–292 (1989). https://doi.org/10.1007/BF00199217

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  • DOI: https://doi.org/10.1007/BF00199217

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