Summary
A murine osteosarcoma in which the number of tumor cells can be continually monitored by measuring the circulating plasma alkaline phosphatase levels was used to determine the effect of tumor burden on peripheral blood and splenic lymphocyte response to mitogens. In animals with tumors of different sizes, the pattern of response of the peripheral blood lymphocytes (PBL) to mitogens is different from that of splenic lymphocytes. PBL response to ConA, PHA, and LPS was initially depressed, but response to PHA and LPS recovered later, as the tumor burden exceeded 6%. However, the recovery of LPS response was not consistent, in that recovery was not seen when the tumor burden was 5%–6%. Response to ConA remained depressed. Splenic lymphocytes showed progressive decline of PHA response. Treatment of tumor-bearing mice with 2-mercaptoethanol (2-ME) restored the ConA response of PBL in 56% of mice. Treatment with 2-ME did not restore PBL response to PHA or LPS.
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Abraham C, Tal Y, Gershon H (1977) Reduced in vitro response to concanavalin A and lipopolysaccharide in senescent mice: A function of reduced number of responding cells. Eur J Immunol 7:301
Adler WH, Takiguchi T, Smith RT (1971) Phytohemagglutinin unresponsiveness in mouse spleen cells induced by methylcholanthrene sarcomas. Cancer Res 31:864
Bluestone JA, Lopez C (1979) Suppression of the immune response in tumor-bearing mice. I. Non-virus-producing tumor cells. J Natl Cancer Inst 63:1215
Elkilali MM, Britton S, Brosman S, Fahey JL (1976) Critical evaluation of lymphocyte functions in urological cancer patients. Cancer Res 36:132
Foad BSI, Adams LE, Yamauchi Y, Litwin A (1974) Phytomitogen responses in peripheral blood lymphocytes in young and older subjects. Clin Exp Immunol 17:657
Ghanta VK, Hiramoto RN, Weiss AB, Caudill L (1976) Monitoring of murine osteosarcoma by serial alkaline phosphatase determinations. J Natl Cancer Inst 57:837
Glaser M, Kirchner H, Herberman RB (1975) Inhibition of in vitro lymphocyte proliferative responses to tumor-associated antigens by suppressor cells from rat bearing progressively growing gross leukemia virus-induced tumors. Int J Cancer 16:384
Heidrick ML, Albright JW, Makinodan T (1980) Restoration of impaired immune functions in aging animals. IV. Action of 2-mercaptoethanal in enhancing age reduced immune responsiveness. Mech Ageing Dev 13:367
Hiramoto RN, Ghanta VK, Soong S-J, Hurst DC (1977) An experimental approach to relate a tumor-associated enzyme marker to tumor cell numbers. Cancer Res 37:365
Hoffeld JT, Oppenheim JJ (1980) Enhancement of the primary antibody response by 2-mercaptoethanol is mediated by its action on glutathione in the serum. Eur J Immunol 10:391
Makinodan T (1979) Restoration of impaired immune functions in aging animals. III. Effects of mercaptoethanol in enhancing the reduced primary antibody responsiveness in vivo. Mech Ageing Dev 11:1
Santer V, Mastromarino JH, Lala PK (1980) Characterization of lymphocyte subsets in spontaneous mouse mammary tumors and host lymphoid organs. Int J Cancer 25:159
Sterling N (1979) Lymphocyte transformation to phytohaem-agglutinin and delayed hypersensitivity related to age and previous cancer history. Clin Exp Immunol 37:352
Varesio L, Govarelli M, Landolfo S, Forni G (1979) Suppression of proliferative response and lymphokine production during the progression of a spontaneous tumor. Cancer Res 39:4983
Whitney RB, Levy JG, Smith AG (1974) Influence of tumor size and surgical resection on cell-mediated immunity in mice. J Natl Cancer Inst 53:111
Zembela M, Myter B, Popiela T, Asherson GL (1977) Depressed in vitro peripheral blood lymphocytes response to mitogens in cancer patients: The role of suppressor cells. Int J Cancer 19:605
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Abbreviations used in this paper: PBL, peripheral blood lymphocytes; peripheral blood lymphocytes; ConA, concanavalin A; PHA, phytohemagglutinin; LPS, lipopolysaccharide; 2-ME, 2-mercaptoethanol; FCS, fetal calf serum; AP, alkaline phosphatase; OS, osteosarcoma
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Kikuchi, Y., Hiramoto, R.N. & Ghanta, V.K. Mitogen response of peripheral blood and splenic lymphocytes and effect of 2-mercaptoethanol in tumor-bearing mice. Cancer Immunol Immunother 12, 225–230 (1982). https://doi.org/10.1007/BF00199178
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DOI: https://doi.org/10.1007/BF00199178