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Immunohistochemical phenotyping of human solid tumors with monoclonal antibodies in devising biotherapeutic strategies

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Summary

A panel of 14 monoclonal antibodies (MoAbs) (4 raised against breast cancer, 6 against colon cancer and 4 against melanoma) were used to phenotype frozen sections of tumor biopsies obtained from 110 patients, by avidin-biotin-peroxidase complex techniques. We observed heterogeneity of antigen expression among the multiple metastatic lesions of single patients, as well as among tumor lesions from different patients with similar tumor histotypes. A wide range of cross-reactivity of anti-(breast-carcinoma) and anti-(colon-carcinoma) MoAbs with other carcinoma histotypes and limited reactivity with melanoma and sarcoma was detected. Some of our anti-melanoma MoAbs were also found to cross-react with selected carcinomas. Nine of the 14 MoAbs most reactive with carcinomas of diverse histotypes have been identified. A mixture or ‘cocktail’ of different MoAbs could be selected for each individual patient in order to achieve binding of MoAbs with most, if not 100% of tumor cells. This study illustrates the approach that we have taken to individualize the cocktail of MoAbs for the development of patient-specific therapeutic immunoconjugates.

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Authors and Affiliations

  1. Biotherapeutics, Inc. and Biological Therapy Institute, 37064, Franklin, TN, USA

    Shuen-Kuei Liao, Connie Meranda, Barry P. Avner, Tammy Romano, Salah Husseini, Bobby Kimbro & Robert K. Oldham

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  1. Shuen-Kuei Liao
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  2. Connie Meranda
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Liao, SK., Meranda, C., Avner, B.P. et al. Immunohistochemical phenotyping of human solid tumors with monoclonal antibodies in devising biotherapeutic strategies. Cancer Immunol Immunother 28, 77–86 (1989). https://doi.org/10.1007/BF00199106

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  • DOI: https://doi.org/10.1007/BF00199106

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