Abstract
High avidity, pentameric, single-domain antibodies, oligomerized through the B subunit of verotoxin, are excellent immunohistochemical reagents. The resulting molecules are termed pentabodies. Here, we describe the immunostaining of tissue sections with ES1, a pentabody recognizing CEACAM6 which is overexpressed in several cancers. The advantages of pentabodies, compared to conventional antibody reagents, in immunohistochemical studies are highlighted.
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Acknowledgments
We thank Jianbing Zhang for providing the rabbit anti-verotoxin antiserum. The authors declare no financial conflict of interest.
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Maik, K.T., MacKenzie, C.R. (2012). Application of Single-Domain Antibodies in Tumor Histochemistry. In: Saerens, D., Muyldermans, S. (eds) Single Domain Antibodies. Methods in Molecular Biology, vol 911. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-968-6_33
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DOI: https://doi.org/10.1007/978-1-61779-968-6_33
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-968-6
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