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Synthesis and properties of glutathione reductase in stressed peas

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Abstract

We have subjected peas (Pisum sativum L.) to four different oxidative stresses: cold conditions (4 °C) in conjunction with light, treatment with paraquat, fumigation with ozone, and illumination of etiolated seedlings (greening). In crude extracts of leaves from stressed plants, an increase (up to twofold) in activity of glutathione reductase (GR) was observed which was consistent with previous reports from several laboratories. In all cases, except for ozone fumigation, the increase in activity was not due to an elevation in the steady-state levels of GR protein. None of the applied stresses had any effect on steady-state levels of GR mRNA. In contrast to the small increase in GR activity, the K m of GR for glutathione disulphide showed a marked decrease when determined for extracts of stressed leaves, compared with that from unstressed plants. This indicates that GR from stressed plants has an increased affinity for glutathione disulphide. The profile of GR activity bands fractionated on non-denaturing acrylamide gels varied for extracts from differently stressed leaves and when compared with GR from unstressed plants. The changes in GR-band profiles and the alteration in the kinetic properties are best explained as changes in the isoform population of pea GR in response to stress.

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Abbreviations

GR:

glutathione reductase

GSSG:

glutathione disulphide

Rubisco:

Ribulose-1,5-bisphosphate carboxylase-oxygenase

RNase A/T1:

ribonucleases A and T1

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Author notes

  1. Corine Enard

    Present address: Laboratoire de Pathologie Vegetale, Institut National Agronomique, 16 rue Claude Bernard, F-75231, Paris Cedex 05, France

Authors and Affiliations

  1. John Innes Centre for Plant Science Research, John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

    E. Anne Edwards, Corine Enard, Gary P. Creissen & Philip M. Mullineaux

Authors
  1. E. Anne Edwards
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  2. Corine Enard
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  3. Gary P. Creissen
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  4. Philip M. Mullineaux
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Additional information

We are grateful to Prof. Alan Wellburn and Dr. Phil Beckett (Division of Biological Sciences, University of Lancaster, UK) for providing ozone-fumigated material and Dr. Jeremy Harbinson for providing material grown at 4° C. This work was supported by a grant-in-aid to the John Innes Institute from the Agricultural and Food Research Council. E.A.E. and C.E. gratefully acknowledge the support of a John Innes Foundation studentship and a European Molecular Biology Organisation Fellowship respectively.

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Edwards, E.A., Enard, C., Creissen, G.P. et al. Synthesis and properties of glutathione reductase in stressed peas. Planta 192, 137–143 (1993). https://doi.org/10.1007/BF00198704

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  • DOI: https://doi.org/10.1007/BF00198704

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