Summary
Generative cells in pollen of Phoenix dactylifera had a convoluted surface and a small cytoplasmic volume, and condensed chromatin in the nucleus. Treatment of hydrated pollen with pectinase followed by grinding in a hypotonic medium released the generative cells from pollen. Convolutions of the plasma-membrane surface in vivo were retained by generative cells in vitro. Generative cells had a much lower solute concentration than did vegetative cells, a property that explains why hypotonic shock was effective in releasing intact generative cells from pollen.
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Southworth, D., Morningstar, P.A. Isolation of generative cells from pollen of Phoenix dactylifera . Sexual Plant Reprod 5, 270–274 (1992). https://doi.org/10.1007/BF00197377
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DOI: https://doi.org/10.1007/BF00197377