Abstract
Deletions of the short arm of chromosome 3 (3p) have been recognized as characteristic features of clear cell renal cell carcinomas (clear cell RCC). We analysed 55 clear-cell RCCs and 30 non-clear-cell kidney tumours (10 papillary and 7 chromophobic RCCs, 11 oncocytomas and 2 collecting duct carcinomas) in loss of heterozygosity (LOH) studies using microsatellite markers for previously observed regions of common deletions on 3p in kidney tumours (3p25, 3p21.3, 3p14.2 and 3p12-13). Alterations were found in all 55 cases of clear-cell RCCs at two to four of the 3p regions. Extensive losses were not found in non-clear-cell tumours except for collecting duct carcinomas; 1 of 10 papillary RCCs showed interstitial deletion limited to a single 3p21.3 locus. LOH analyses using microsatellite markers for regions of common deletions at 3p may be of value in differential diagnosis of kidney tumours.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anglard P, Tory K, Brauch H, Weiss GH, Latif F, Merino MJ, Lerman MI, Zbar B, Linehan WM (1991) Molecular analysis of genetic changes in the origin and development of renal cell carcinoma. Cancer Res 51:1071–1077
Anglard P, Trahan E, Liu S, Latif F, Merino MJ, Lerman MI, Zbar B, Linehan WM (1992) Molecular characterization of human renal cell carcinoma cell lines. Cancer Res 52: 348–356
Cawkwell L, Bell SM, Lewis FA, Dixon MF, Taylor GR, Quirke P (1993) Rapid detection of allele loss in colorectal tumours using microsatellites and fluorescent DNA technology. Br J Cancer 67:1262–1267
El-Naggar A, Batsakis J, Wang G, Lee M-S (1993) PCR-based RELP screening of the commonly deleted 3p loci in renal cortical neoplasms. Diagn Mol Pathol 2:269–276
Foster K, Prowse A, Berg A van den, Fleming S, Hulsbeek MMF, Crossey PA, Richards FM, Cairns P, Affara NA, Ferguson-Smith MA, Buys CHCM, Maher ER (1994) Somatic mutations of the von Hippel-Lindau disease tumour sppressor gene in nonfamilial clear-cell renal carcinoma. Hum Mol Genet 3:2169–2173
Füzesi L, Cober M, Mittermayer C (1992) Collecting duct carcinoma: cytogenetic characterization. Histopathology 21:155–160
Gnarra JR, Tory K, Weng Y, Schmidt L, Wei MH, Li H, Latif F, Liu S, Chen F, Duh F-M, Lubensky I, Duan DR, Florence C, Pozzatti R, Walther MM, Bander NH, Grossman HB, Brauch H, Pomer S, Brooks JD, Issacs WB, Lerman MI, Zbar B, Linehan WM (1994) Mutations of the VHL tumour suppressor gene in renal carcinoma. Nat Genet 7:85–90
Kovacs G, Erlandsson R, Boldog F, Ingvarsson S, Muller-Brechlin R, Klein G, Sumegi J (1988) Consistent chromosome 3p deletion and loss of heterozygosity in renal cell carcinoma. Proc Natl Acad Sci USA 85:1571–1575
Kovacs A, Störkel S, Thoenes W, Kovacs G (1992) Mitochondrial and chromosomal DNA alterations in human chromophobe renal cell carcinoma. J Pathol (Lond) 167:273–277
LaForgia S, Lasota J, Latif F, Boghosian-Sell L, Kastury K, Ohta M, Druck T, Atchison L, Cannizzaro L, Barnea G, Schlesinger J, Modi W, Kuzmin I, Tory K, Zbar B, Croce CM, Lerman M, Huebner K (1993) Detailed genetic and physical map of the 3p chromosome region surrounding the familial RCC chromosome translocation, t(3;8)(p14.2;q24.1). Cancer Res 53:3118–3124
Lubinski J, Chosia M, Kotanska K, Huebner K (1988) Genotypic analysis of DNA isolated from fine needle aspiration biopsies. Anal Quant Cytol Histol 10:383–390
Lubinski J, Hadaczek P, Podolski J, Toloczko A, Sikorski A, McCue P, Druck T, Huebner K (1994) Common regions of deletion in chromosome regions 3p12 and 3p14.2 in primary clear-cell renal carcinomas. Cancer Res 54:3710–3713
Morita R, Ishikawa J, Tsutsumi M, Hikiji K, Tsukada Y, Kamidono S, Maeda S, Nakamura Y (1991) Allelotype of renal cell carcinoma. Cancer Res 51:820–823
Naylor S, Buys CHCM, Carritt B (1994) Report of the Fourth International Workshop on Human Chromosome 3 Mapping. Cytogenet Cell Genet 65:1–50
Ogawa O, Kakehi Y, Ogawa K, Koshiba M, Sugiyama T, Yoshida O (1991) Allelic loss at chromosome 3p characterizes clear-cell phenotype renal cell carcinoma. Cancer Res 51:949–953
Presti J, Reuter V, Cordon-Cordo C, Mazumdar M, Fair WR, Jhanwar SC (1993) Allelic deletions in renal tumours: histopathological correlations. Cancer Res 53:5780–5783
Robson C, Churchil B, Anderson W (1969) The results of radical nephrectomy for renal cell carcinomas. J Urol 101:297–301
Roche J, Whisenant E, Boldog F, Loeb D, Vance JM, Drabkin H (1994) Dinucleotide repeats flanking the renal cell carcinoma breakpoint at 3p14.2. Hum Mol Genet 3:215
Tallini G (1994) Analysis of nuclear and mitochondrial DNA alterations in thyroid and renal oncocytic tumour. Cytogenet Cell Genet 66:250–259
Thoenes W, Störkel S, Rumpelt H (1986) Histopathology and classification of renal cell tumours (adenomas, oncocytomas and carcinomas): the basic cytological and histopathological elements and their use for diagnosis. Pathol Res Pract 181:125–143
Van den Berg A, Hulsbeek MMF, Jong D de, Kok K, Veldhuis PMJF, Roche J, Buys CHCM (1996) Major role for a 3p21 region and lack of involvement of the t(3;8) breakpoint region in the development of renal cell carcinoma suggested by loss of heterozygosity analysis. Genes Chromosome Cancer 15:64–72
Van den Berg E, Hout A van der, Oosterhuis J, Störkel S, Dijkhuizen T, Dam A, Zweers HMM, Mensink HJA, Buys CHCM, Jong B de (1993) Cytogenetics analysis of epithelial renal-cell tumours: relationship with a new histopathological classification. Int J Cancer 55:223–227
Van der Hout AH, Berg E van den, Vlies P van der, Dijkhuizen T, Störkel S, Oosterhuis JW, Jong B de, Buys CHCM (1993) Loss of heterozygosity at the short arm of chromosome 3 in renal-cell cancer correlates with the cytological tumour type. Int J Cancer 53:353–357
Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AMM, Bos JL (1988) Genetic alterations during colorectal-tumour development. N Engl J Med 319:525–532
Yamakawa K, Morita R, Takahashi E, Hori T, Ishikawa J, Nakamura Y (1991) A detailed deletion mapping of the short arm of chromosome 3 in sporadic renal cell carcinoma. Cancer Res 51:4707–4711
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hadaczek, P., Podolski, J., Toloczko, A. et al. Losses at 3p common deletion sites in subtypes of kidney tumours: histopathological correlations. Vichows Archiv A Pathol Anat 429, 37–42 (1996). https://doi.org/10.1007/BF00196818
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00196818