Summary
Locomotor activity of the river lamprey, Lampetra japonica, was investigated under a light-dark (LD 12∶12) cycle and under continuous dark conditions. Intact lampreys were entrained to the light:dark cycle. They were active mainly in the early half of the dark period and inactive in light period. The light:dark entrainment continued in 72.7% of lampreys after the removal of bilateral eyes, but additional pinealectomy made the entrainment disappear in all lampreys. When lampreys were pinealectomized with their eyes intact, light: dark entrainment was abolished in most cases. The results indicate that the pineal organ of the lamprey is a photoreceptive organ responsible for synchronizing locomotor activity to LD cycle. Under continuous dark conditions, the locomotor activity began to free-run with a period of 21.3 ± 0.9 h (mean ± SD, n = 53). This circadian rhythmicity was not affected by the removal of lateral eyes but was abolished by pinealectomy. The pineal organ appears to function as an oscillator, or as one of the oscillators, for the circadian locomotor rhythm of lampreys.
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Abbreviations
- DD:
-
continuous dark
- LD:
-
light:dark
References
Cole WC, Youson JH (1982) Morphology of the pineal complex of the anadromous sea lamprey, Petromyzon marinus L. Am J Anat 165:131–163
Collin JP (1969) Contribution à l'étude de l'organe pinéal. De l'épiphyse sensorielle à la glande pinéale: modalités de transformation et implications fonctionnelles. Ann Stn Biol Besse-enChandesse [Suppl] 1:1–359
Deguchi T (1979) Role of adenosine 3′,5′-monophosphate in the regulation of circadian oscillation of serotonin N-acetyltransferase activity in cultured chicken pineal gland. J Neurochem 33:45–51
Dickson DH, Graves DA (1981) The ultrastructure and development of the eye. In: Hardisty MW, Potter IC (eds) The biology of lampreys, vol 3. Academic Press, London New York, pp 43–94
Ebihara S, Kawamura H (1981) The role of the pineal organ and the suprachiasmatic nucleus in the control of circadian locomotor rhythms in the Java sparrow, Padda oryzivora. J Comp Physiol 141:207–214
Ebihara S, Uchiyama K, Oshima I (1984) Circadian organization in the pigeon, Columba livia: the role of the pineal organ and the eye. J Comp Physiol A 154:59–69
Gaston S, Menaker M (1968) Pineal function: the biological clock in the sparrow? Science 160:1125–1127
Hardisty MW, Potter IC (1971a) The behavior, ecology and growth of larval lampreys. In: Hardisty MW, Potter IC (eds) The biology of lampreys, vol 1. Academic Press, London New York, pp 85–125
Hardisty MW, Potter IC (1971b) General biology of adult lampreys. In: Hardisty MW, Potter IC (eds) The biology of lampreys, vol 1. Academic Press, London New York, pp 127–206
Kavaliers M (1979) Pineal involvement in the control of circadian rhythmicity in the lake chub, Couesius plumbeus. J Exp Zool 209:33–40
Kavaliers M (1980) Circadian locomotor activity rhythm of the burbot, Lota lota: seasonal differences in period length and the effect of pinealectomy. J Comp Physiol 136:215–218
McMillan JP (1972) Pinealectomy abolishes the circadian rhythm of migratory restlessness. J Comp Physiol 79:105–112
Morita Y (1975) Direct photosensory activity of the pineal. In: Knigge KM, Scott DE, Kobayashi H, Ishii S (eds) Brain-endocrine interaction, vol 2. The ventricular system. Karger, Basel, pp 376–387
Morita Y, Dodt E (1971) Photosensory responses from the pineal eye of the lamprey (Petromyzon fluviatilis). Proc IUPS, vol 9, p 405
Morita Y, Dodt E (1973) Slow photic responses of the isolated pineal organ of lamprey. Nova Acta Leopold 38:331–339
Morita Y, Tabata M, Tamotsu S (1985) Intracellular response and input resistance change of pineal photoreceptors and ganglion cells. Neurosci Res [Suppl] 2:S79-S88
Morita Y, Samejima M, Tamotsu S (1989) Response patterns and neuronal networks of photosensory pineal organs. Arch Histol Cytol 52 [Suppl]:469–475
Morita Y, Tamotsu S, Uchida K (1989) Multiplicity of electrophysiological and immunocytochemical properties in the pineal photosensory system. In: Reiter RJ, Pang SF (eds) Advances in pineal research 3. J Libbey, London, pp 43–48
Ooka-Souda S, Kabasawa H (1988) Circadian rhythms in locomotor activity of the hagfish, Eptatretus burgeri III. Hypothalamus: a locus of the circadian pacemaker? Zool Sci 5:437–442
Ooka-Souda S, Kabasawa H, Kinoshita S (1985) Circadian rhythms in locomotor activity of the hagfish, Eptatretus burgeri, and the effect of reversal of light-dark cycle. Zool Sci 2:749–754
Pu GA, Dowling JE (1981) Anatomical and physiological characteristics of pineal photoreceptor cell in the larval lamprey, Petromyzon marinus. J Neurophysiol 46:1018–1038
Roberts A (1978) Pineal eye and behavior in Xenopus tadpoles. Nature 273:774–775
Robertson LM, Takahashi JS (1988) Circadian clock in cell culture: I. Oscillation of melatonin release from dissociated chick pineal cells in flow-through microcarrier culture. J Neurosci 8:12–21
Tabata M, Minh-Nyo M (1989) Dual photobehavioral response in catfish. Zool Sci 6:427–431
Tabata M, Minh-Nyo M, Niwa H, Oguri M (1989) Circadian rhythm of locomotor activity in a teleost, Silurus asotus. Zool Sci 6:367–375
Takahashi JS, Menaker M (1984) Multiple redundant circadian oscillators within the isolated avian pineal gland. J Comp Physiol A 154:435–440
Tamotsu S, Morita Y (1986) Photoreception in pineal organs of larval and adult lampreys, Lampetra japonica. J Comp Physiol A 159:1–5
Tamotsu S, Korf H-W, Morita Y, Oksche A (1990) Immunocytochemical localization of serotonin and photoreceptor-specific proteins (rod-opsin, S-antigen) in the pineal complex of the river lamprey, Lampetra japonica, with special reference to photoneuroendocrine cells. Cell Tissue Res 262:205–216
Uchida K, Nakamura T, Morita Y (1992) Signal transmission from pineal photoreceptors to luminosity-type ganglion cells in the lamprey, Lampetra japonica. Neurosci 47:241–247
Underwood H (1983) Circadian pacemakers in lizards: phaseresponse curves and effects of pinealectomy. Am J Physiol 244:R857-R864
Young JZ (1935) The photoreceptors of lampreys. 1. Light-sensitive fibres in the lateral line nerves. J Exp Biol 12:229–238
Zimmerman NH, Menaker M (1979) The pineal gland: a pacemaker within the circadian system of the house sparrow. Proc Natl Acad Sci USA 76:999–1003
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Morita, Y., Tabata, M., Uchida, K. et al. Pineal-dependent locomotor activity of lamprey, Lampetra japonica, measured in relation to LD cycle and circadian rhythmicity. J Comp Physiol A 171, 555–562 (1992). https://doi.org/10.1007/BF00194104
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DOI: https://doi.org/10.1007/BF00194104