Abstract
The cytological and intracellular localization of myrosinase (EC 3.2.3.1) has been studied by immunochemical techniques using paraffin-embedded sections of radicles and cotyledons from seeds of Brassica napus L. cv. Niklas. For immunolabelling, sections were sequentially incubated with a monoclonal anti-myrosinase antibody and with peroxidase-and fluorescein-isothiocyanate-conjugated secondary antibodies. Enzyme and fluorescence label was present in typical myrosin cells both in radicles and in cotyledons. With higher magnification, fluorescence label revealed that the intracellular localization of myrosinase was associated with the tonoplast-like membrane surrounding the myrosin grains in the myrosin cells. The results also indicate that a large proportion of the positive myrosin cells are located in the second-outermost cell layer of the peripheral cortex region of the radicles.
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Abbreviations
- FITC:
-
fluorescein isothiocyanate
- PBS:
-
phosphate-buffered saline
- PBS-T:
-
PBS with 0.5% (v/v) Tween-20 (polyoxyethylene sorbitane monolaurate)
References
Bones, A., Iversen, T.H. (1985) Myrosin cells and myrosinase. Isr. J. Bot. 34, 351–375
Bones, A., Evjen, K., Iversen, T.H. (1989) Characterization and distribution of dilated cisternae of endoplasmic reticulum in intact plants, protoplasts and calli of Brassicaceae. Isr. J. Bot. (in press)
Bones, A.M., Slupphaug, G. (1989) Purification, characterization and partial amino acid sequencing of β-thioglucosidase from Brassica napus L. J. Plant Physiol. 134, 722–729
Guignard, L. (1890a) Recherches sur la localisation des principes actifs des crucifères. J. Bot. 4, 385–394, 412–430, 435–455
Guignard, L. (1890b) Sur la localisation des principes qui fournissent les essences sulfurées des Crucifères. C.R. Acad. Sci. III, 249–251
Heinricher, E. (1884) Über Eiweissstoffe führende Idioblasten bei einigen Cruciferen. Ber. Dtsch. Bot. Ges. II, 463–467
Hoefert, L.L., Martin, S.S. (1980) Ultrastructure of protein-accumulating cells (idioblasts) in Thlaspi arvense. Bot. Soc. Am. Misc. Ser. Publ. 158, 52
Jørgensen, L.B., Behnke, H.-D., Mabry, T.J. (1977) Protein-accumulating cells and dilated cisternae of the endoplasmatic reticulum in three glucosinolate containing genera: Armoracia, Capparis, Drypetes. Planta 137, 215–224
Iversen, T.H. (1970) Cytochemical localization of myrosinase (β- thioglucosidase) in root tips of Sinapis alba. Protoplasma 71, 451–466
Iversen, T.H., Baggerud, C., Beisvåg, T. (1979) Myrosin cells in Brassicaceae roots. Z. Pflanzenphysiol. 97, 143–154
Lüthy, B., Matile, P.H. (1984) The mustard oil bomb: rectified analysis of the subcellular organization of the myrosinase system. Biochem. Physiol. Pflanz. 179, 5–12
Matile, P.H. (1980) ‚'Die Senfölbombe”: Zur Kompartmentierung des Myrosinasesystems. Biochem. Physiol. Pflanz. 175, 722–731
Peche, K. (1913) Mikrochemischer Nachweis des Myrosins. Ber. Dtsch. Bot. Ges. 31, 458–462
Schweidler, J.H. (1905) Die systematische Bedeutung der Eiweissoder Myrosinzellen der Cruciferen nebst Beiträgen zu ihrer anatomisch-physiologischen Kenntnis. Ber. Dtsch. Bot. Ges. 23, 274–285
Spatzier, W. (1893) Über das Aufreten und die physiologische Bedeutung des Myrosins in der Pflanze. Jahrb. Wiss. Bot. 25, 39–79
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This work was supported by The Norwegian Research Council for Science and the Humanities. We wish to thank Professor Med. O.A. Haugen, Department of Pathology, University of Trondheim, Norway, for the skilful assistance provided regarding fixation and sectioning.
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Thangstad, O.P., Iversen, T.H., Slupphaug, G. et al. Immunocytochemical localization of myrosinase in Brassica napus L.. Planta 180, 245–248 (1990). https://doi.org/10.1007/BF00194003
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DOI: https://doi.org/10.1007/BF00194003