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Somatic embryogenesis in cultured immature kernels of Pistachio, Pistacia vera L.

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Abstract

Embryogenic tissue was produced from kernels of immature fruits of Pistachio (Pistacia vera L.) cultured in liquid Murashige and Skoog media, supplemented with 200 mgl−1 casein hydrolysate, 114 μM 1-ascorbic acid, and benzylaminopurine. Compact embryogenic masses differentiated directly from the fruit explants after culture for 2 weeks in liquid medium with 8.9 μM benzylaminopurine. After transfer of the embryogenic masses into the same medium, but with 4.4 μM benzylaminopurine, somatic embryos appeared. Several stages of embryogenesis were present in the cultures. Adventive embryos were readily separated from the friable embryogenic masses by shaking. Separated somatic embryos, germinated on solidified Murashige & Skoog medium without growth regulators, developed into plantlets.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

benzylaminopurine (N6-benzyladenine)

EMS:

embryogenic mass

MS:

Murashige and Skoog medium (Sigma M-0404)

NAA:

α-naphthalene acetic acid

PGR:

plant growth regulator

TDZ:

thidiazuron (1-phenyl-3-(1,2,3, thiadiazol-5-yl)urea)

WP:

McCown's Woody plant medium (Sigma M6774)

ABA:

abscisic acid

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Authors and Affiliations

  1. University of Edinburgh, Institute of Cell and Molecular Biology, Daniel Rutherford Building, King's Buildings, EH9 3JH, Edinburgh, UK

    A. Onay, C. E. Jeffree & M. M. Yeoman

Authors
  1. A. Onay
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  2. C. E. Jeffree
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  3. M. M. Yeoman
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Additional information

Communicated by M. R. Davey

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Onay, A., Jeffree, C.E. & Yeoman, M.M. Somatic embryogenesis in cultured immature kernels of Pistachio, Pistacia vera L.. Plant Cell Reports 15, 192–195 (1995). https://doi.org/10.1007/BF00193718

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  • DOI: https://doi.org/10.1007/BF00193718

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