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Identified nonspiking interneurons in leg reflexes and during walking in the stick insect

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Abstract

In the stick insect Carausius morosus identified nonspiking interneurons (type E4) were investigated in the mesothoracic ganglion during intraand intersegmental reflexes and during searching and walking.

In the standing and in the actively moving animal interneurons of type E4 drive the excitatory extensor tibiae motoneurons, up to four excitatory protractor coxae motoneurons, and the common inhibitor 1 motoneuron (Figs. 1–4).

In the standing animal a depolarization of this type of interneuron is induced by tactile stimuli to the tarsi of the ipsilateral front, middle and hind legs (Fig. 5). This response precedes and accompanies the observed activation of the affected middle leg motoneurons. The same is true when compensatory leg placement reflexes are elicited by tactile stimuli given to the tarsi of the legs (Fig. 6).

During forward walking the membrane potential of interneurons of type E4 is strongly modulated in the step-cycle (Figs.8–10). The peak depolarization occurs at the transition from stance to swing. The oscillations in membrane potential are correlated with the activity profile of the extensor motoneurons and the common inhibitor 1 (Fig. 9).

The described properties of interneuron type E4 in the actively behaving animal show that these interneurons are involved in the organization and coordination of the motor output of the proximal leg joints during reflex movements and during walking.

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Abbreviations

CLP:

reflex, compensatory leg placement reflex

CI1:

common inhibitor I motoneuron

fCO:

femoral chordotonal organ

FETi:

fast extensor tibiae motoneuron

FT:

femur-tibia

SETi:

slow extensor tibiae motoneuron

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Author notes

  1. R. Kittmann

    Present address: Zoologisches Institut I, Universität Freiburg, D-79104, Freiburg, Germany

Authors and Affiliations

  1. FB Biologie, Universität Kaiserslautern, P.O. Box 3049, D-67653, Kaiserslautern, Germany

    A. Büschges & R. Kittmann

  2. Fakultät für Biologie, Universität Bielefeld, P.O. Box 100131, D-33501, Bielefeld, Germany

    J. Schmitz

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  1. A. Büschges
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  2. R. Kittmann
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  3. J. Schmitz
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Büschges, A., Kittmann, R. & Schmitz, J. Identified nonspiking interneurons in leg reflexes and during walking in the stick insect. J Comp Physiol A 174, 685–700 (1994). https://doi.org/10.1007/BF00192718

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