Abstract
The genes encoding for six receptors involved in the proinflammatory response lie on different chromosomes. Two receptors for N-formylpeptides (FPR1, FPR2), one homologue of these (FPRL2), and the receptor for complement fragment C5a (C5aR) are encoded by four genes mapped to human chromosome 19. The genes encoding two receptors for Interleukin-8 (IL8RA, IL8RB) have been located on human chromosome 2. In this report we describe the physical linkage between these genes in two different clusters. DNA fragments obtained by digestion with several restriction enzymes were separated by pulsed field gel electrophoresis. Nylon filters were hybridized with probes corresponding to the complete translated sequences of these genes. These probes were obtained from a human neutrophil cDNA-library. The four genes on chromosome 19 are contained in a 200 kilobase (kb) fragment. Both Interleukin-8 receptors are on a 150 kb fragment. The complete translated sequences for these genes were amplified from genomic DNA, indicating that they are contained in a single exon.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahuja, S. K., Ozcelik, T., Milatovitch, A., Francke, U., and Murphy, P. M. Molecular Evolution of the human interleukin-8 receptor gene cluster. Nature Genetics 2: 31–36, 1992
Baggiolini, M. and Clark-Lewis, I., Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett 307: 97–101, 1992
Bao, L., Gerard, N. P., Eddy, R. L., Shows, T. B., and Gerard, C. Mapping of the genes for the human C5a receptor (C5AR), human FMLP receptor, and two FMLP receptor homologue orphan receptors (FPRH1, FPRH2) to chromosome 19. Genomics 13: 437–440, 1992
Boulay, F., Tardif, M., Brouchon, L., and Vignais, P. Human N-Formylpeptide Receptor. Characterization of the Two CDNA isolates and evidence for a new subfamily of G-protein-coupled receptors. Biochemistry 29: 11123–11133, 1990
De Nardin, E., Rade, S. J., Lewis, N., Genco, R. J., and Hamrnarskjold, M. Idenfification of a gene encoding for the human formyl peptide receptor. Biochem Int 26: 381–387, 1992
Doolittle, R. F. and Feng, D.-F. Nearest neighbor procedure for relating progressively aligned amino acid sequences. Methods Enzymol 183: 659–669, 1990
Gerard, N. P. and Gerard, C. The chemotactic receptor for human C5a anaphylotoxin. Nature 349: 614–617, 1991
Gerard, C., Bao, L., Orozco, O., Pearson, M., Kunz, D., and Gerard, N. P. Structural diversity in the extracellular faces of peptidergic G-protein-coupled receptors. J Immunol 149: 2600–2606, 1993
Holmes, W. E., Lee, J., Kuang, W.-J., Rice, G. C., and Wood, W. I. Structure and functional expression of a human interleukin-8 receptor. Science 253: 1278–1280, 1991
Hugli, T. E. The structural basis for anaphylactoxin and chemotactic functions of C3a, C4a, and C5a. Crit Rev Immunol 1: 321–366, 1981
Lloyd, A., Modi, W., Srenger, H., Cevario, S., Oppenheim, J., and Kelvin, D. Assignment of genes for interleukin-8 receptors (IL8R) A and B to human chromosome band 2q35. Cytogenet Cell Genet 63: 238–240, 1993
Maniatis, T., Fristch, E. F., and Sambrook, J. Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989
Morris, S. W., Nelson, N., Valentine, M. B., Shapiro, D. N., Look, A., Zozlosky, C. J., Beckmann, M. P., and Cerreti, D. P. Assignment of the genes encoding human Interleukin 8 receptor types I and II and Interleukin 8 receptor psudogene to chromosome 2q35. Genomics 14: 685–691, 1992
Mullis, K. B. and Faloona, F. A. Specific synthesis of DNA in vitro via polymerase-catalyzed chain reaction. Meth Enzymol 155: 355, 1987
Murphy, P. M. and Tiffany, H. L. Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science 253: 1280–1282, 1991
Murphy, P. M., Ozceklik, T., Kenney, R. T., Tiffany, H. L., Mcdermott, D., and Franche, U. A structural homologue of the N-formyl peptide receptor: characterization and chromosome mapping of a peptide chemoattractant. J Biol Chem 267: 7637–7643, 1992
Neote, K., DiGregorio, D., Mak, J. Y. Horuk, R., and Schall, T. Molecular cloning, functional expression and signaling characteristics of a C-C chemokin receptor. Cell 72: 415–425, 1993
Oppenheim, J. J., Zachariae, C. O. C., Mukaida, N., and Matsuschima, K. Properties of the novel proinflammatory supergene “intercrine” cytokine family. Annu Rev Immunol 9: 617–648, 1991
Sanger, F., Nicklen, S., and Coulson, A. B. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977
Smith, C. L., Klco, S. R., and Cantor, C. R. Pulsed field gel electrophoresis and the technology of large DNA molecules. In K. E. Davies (ed.): Genome Analysis: A Practical Approach, 41–72, IRL, Oxford, 1988
Snyderman, R. and Goetzl, E. J. Molecular and cellular mechanism of leukocyte chemotaxis. Science 213: 830, 1981
Ye, R. D., Cavanagh, S. L., Quehenberger, O., Prossnitz, E. R., and Cochrane, C. G. Isolation of CDNA that encodes a novel granulocyte N-Formyl Peptide Receptor. Biochem Biophys Res Commun 184: 582–589, 1992
Author information
Authors and Affiliations
Additional information
The contributions of the first two authors to this research was equal
Rights and permissions
About this article
Cite this article
Alvarez, V., Coto, E., Setién, F. et al. A physical map of two clusters containing the genes for six proinflammatory receptors. Immunogenetics 40, 100–103 (1994). https://doi.org/10.1007/BF00188171
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00188171