Abstract
Pellets of Tyromyces sambuceus were cultured in standard liquid media that contained a natural triacylglycerol as an additional carbon source. Parallel to a drop of pH below 2.0 and the formation of R(+)-4-decanolide, the oil droplets disappeared. Assays based on 3-naphthyl ester showed that most of the lipolytic and esterolytic enzyme activities were membrane-associated. The time course of enzyme activities after addition of castor oil suggested an inducibility of both membrane-bound and intracellular lipases, while only the intracellular esterases responded significantly. The results could contribute to improving the biotechnological generation of lipid-derived flavours and other compounds.
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Hädrich-Meyer, S., Berger, R.G. Localization of lipolytic and esterolytic activities of Tyromyces sambuceus, a 4-decanolide-producing basidiomycete. Appl Microbiol Biotechnol 41, 210–214 (1994). https://doi.org/10.1007/BF00186961
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DOI: https://doi.org/10.1007/BF00186961