Abstract
Anaerobic thermophilic degradation of several amino acids was studied in batch cultures using an inoculum from a steady-state semicontinuous enrichment culture. Experiments were done in the presence and absence of methanogenesis and known electron acceptors in the Stickland reaction. Methanogenesis was found to be crucial for the degradation of amino acids known to be oxidatively deaminated (leucine, valine and alanine). Other amino acids (serine, threonine, cysteine and methionine) were degraded under both methanogenic and non-methanogenic conditions. Degradation rates for these four amino acids were 1.3 to 2.2 times higher in cases where methanogenesis was active. The degradation rates of serine, threonine, cysteine and methionine were about twice as high as the rates of leucine, valine and alanine under methanogenic conditions. Inclusion of different electron acceptors, known to work in the Stickland reaction, did not enhance the degradation rates of any amino acid used nor did they alter the degradation patterns. Glycine was oxidatively deaminated to acetate, carbon dioxide, hydrogen and ammonium.
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References
Andreesen JR, Bahl H, Gottschalk G (1989) Introduction to the physiology and biochemistry of the genus Clostridium, In: Minton NP, Clarke DJ (eds) Clostridia. Plenum, New York, pp 27–62
Bader J, Rauschenbach P, Simon H (1982) On a hitherto unknown fermentation path of several amino acids by proteolytic Clostridia. FEBS Lett 140:67–72
Barker HA (1981) Amino acid degradation by anaerobic bacteria. Annu Rev Biochem 50:23–40
Barker HA, Wiken T (1948) The origin of butyric acid in the fermentation of threonine by Clostridium aminovalericum. Arch Biochem 17:149–151
Buckel W (1991) Ungewöhnliche Chemie bei der Fermentation von Aminosäuren durch anaerobe Bakterien. Bioforum 14:7–19
Cardon BP, Barker HA (1947) Amino acid fermentation by Clostridium propionicum and Diplococcus glycinophilus. Arch Biochem 12:165–180
Dean JA (1973) Lange's handbook of chemistry, 11th edn. McGraw-Hill, New York
Durre P, Andeesen JR (1982) Selenium-dependent growth and glycin fermentation by Clostridium purinolyticum. J Gen Microbiol 128:1457–1466
Elsden SR, Hilton MG (1978) Volatile acid production from threonine, valine, leucine and isoleucine by Clostridia. Arch Microbiol 117:165–172
Erikson LE (1980) Biomass elemental composition and energy content. Biotechnol Bioeng 22:451–456
Golovchenko NP, Belokopytov BF, Akimenko VK (1982) Threonine catabolism in the bacterium Clostridium sticklandii. Biochemistry Engl Trans Biokhimiya 47:969–974
Gottschalk G (1986) Bacterial Metabolism, 2nd edn. Springer Verlag, New York
Guangsheng C, Plugge CM, Roelofsen W, Houwen FP, Stams AJM (1992) Selenomonas acidaminovorans sp. nov., a versatile thermophilic proton-reducing anaerobe with the ability to grow by decarboxylation of succinate to propionate. Arch Microbiol 157:169–175
Hino T, Russell JB (1985) Effect of reducing-equivalent disposal and NADH/NAD on deamination of amino acids by intact rumen microorganisms and their cell extracts. Appl Environ Microbiol 50:1368–1374
Ianotti EL, Kafkewitz D, Wolin MJ, Bryant MP (1973) Glucose fermentation products of Ruminococcus albus grown in continuous culture with Vibrio succinogenes: changes caused by interspecies transfer of H2. J Bacteriol 114:1231–1240
Kiene RP, Malloy KD, Taylor BF (1990) Sulphur-containing amino acids as precursors of thiols in anoxic coastal sediments. Appl Environ Microbiol 56:156–161
Lewis D, Elsden SR (1955) The fermentation of l-threonine, l-serine, l-cysteine and acrylic acid by a gram-negative coccus. Biochem J 60:683–691
McInerney MJ (1988) Anaerobic hydrolysis and fermentation of fats and proteins, In: Zehnder AJB (ed.) Biology of anaerobic microorganisms. Wiley, New York Chichester Brisbane pp 373–415
Nagase M, Matsuo T (1982) Interaction between amino-acid-degrading bacteria and methanogenic bacteria in anaerobic digestion. Biotechnol Bioeng 24:2227–2239
Nanninga HJ, Gottschal JC (1985) Amino acid fermentation and hydrogen transfer in mixed cultures. FEMS Microbiol Ecol 31:261–269
Örlygsson J (1994) The role of interspecies hydrogen transfer on thermophilic protein and amino acid metabolism. PhD Thesis. Swedish University of Agricultural Sciences
Örlygsson J, Houwen FP, Svensson BH (1993) Anaerobic degradation of protein and the role of methane formation in steady state thermophilic enrichment cultures. Swed J agric Sci 23:45–54
Örlygsson J, Houwen FP, Svensson BH (1994) Influence of hydrogenotrophic methane formation on the thermophilic anaerobic degradation of protein and amino acids. FEMS Microbiol Ecol 13:327–334
Peterson GL (1983) Determination of total protein. Methods Enzymol 91:95–119
Russell JB, Jeraci JL (1984) Effect of carbon monoxide on fermantation of fiber, starch, and amino acids by mixed rumen microorganism in vitro. Appl Environ Microbiol 48:211–217
Ruiz-Herrera J, Starkey RL (1969) Dissimilation of methionine by fungi. J Bacteriol 99:544–551
Rutgers M, Van Dam K, Westerhoff HV (1991) Control and thermodynamics of microbial growth: rational tools for bioengineering. Crit Rev Biotechnol 11:367–395
Segal W, Starkey RL (1969) Microbial decomposition of methionine and identification of the resulting sulphur products. J Bacteriol 98:908–913
Seto B (1980) The Stickland reaction, In: Knowles CJ (ed.) Diversity of bacterial respiratory systems. CRC, Boca Raton, Fla, pp 50–64
Stams AJM, Hansen TA (1984) Fermentation of glutamate and other compounds by Acidaminobacter hydrogenoformans gen. nov. sp. nov., an obligate anaerobe isolated from black mud. Studies with pure cultures and mixed cultures with sulphate-reducing and methanogenic bacteria. Arch Microbiol 137:329–337
Stickland LH (1934) Studies in the metabolism of the strict anaerobes (genus Clostridium). I. The chemical reactions by which Cl. sporogenes obtains its energy. Biochem J 28:1746–1759
Thauer RK, Jungerman K, Decker K (1977) Energy conservation in chemothrophic anaerobic bacteria. Bacteriol Rev 41:100–180
Tokushigo M, Hayaishi O (1972) Threonine metabolism and its regulation in Clostridium tetanomorphum. J Biochem (Tokyo) 72:469–477
Wiesendanger S, Nisman S (1953) La l-méthionine démercaptodésaminase: un nouvel enzyme à pyridoxal-phosphate. Acad Sci 237:764–765
Wildenauer FX, Winter J (1986) Fermentation of isoleucine and arginine by pure and syntrophic cultures of Clostridium sporogenes. FEMS Microbiol Ecol 38:373–379
Winter J, Schindler F, Wildenauer FX (1987) Fermentation of alanine and glycine by pure and syntrophic cultures of Clostridium sporogenes. FEMS Microbiol Ecol 45:153–161
Wolin MJ, Miller TL (1982) Interspecies hydrogen transfer: 15 years later. ASM News 48:561–565
Woods DD, Clifton CE (1937) Studies in the metabolism of the strict anaerobes (genus Clostridium). VI. Hydrogen production and amino acid utilization by Clostridium tetanomorphum. Biochem J 31:174–178
Woods DD, Trim R (1942) The metabolism of amino acids by Clostridium welchii. Biochem J 35:501–507
Zehnder AJB, Huser BA, Brock TA, Wuhrmann K (1980) Characterization of an acetate-decarboxylating, non-hydrogen-oxidizing methane bacterium. Arch Microbiol 124:1–11
Zindel U, Freudenberg W, Rieth M, Andreesen JR, Schnell J, Widdel F (1988) Eubacterium acidaminophilum sp. nov., a versatile amino acid-degrading anaerobe producing or utilizing H2 or formate. Arch Microbiol 150:254–266
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Örlygsson, J., Houwen, F.P. & Svensson, B.H. Thermophilic anaerobic amino acid degradation: deamination rates and end-product formation. Appl Microbiol Biotechnol 43, 235–241 (1995). https://doi.org/10.1007/BF00172818
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DOI: https://doi.org/10.1007/BF00172818