Summary
A cDNA clone, λLPO-1, encoding a major lignin peroxidase from the basidiomycete Bjerkandera adusta was isolated and characterized. The nucleotide sequence of λLPO-1 predicts a mature protein consisting of 349 amino acids with a molecular weight of 37,225 preceded by a signal peptide of 23 amino acid residues. We have also cloned and sequenced the gene encoding lignin peroxidase from B. adusta. Comparison of these sequences reveals a lignin peroxidase gene structure consisting of 1,116 bp of protein-encoding DNA that is interrupted by four intervening sequences. The putative eukaryotic regulatory sequence, a TATA box, is present at position — 75 relative to the translational initiation codon. Amino acid sequence homology between the coding regions of λLPO-1 and of the lignin peroxidase cDNA clone λML-1 from Phanerochaete chrysosporium is 61%.
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References
Andrawis A, Pease EA, Kuan I-C, Holzbaur E, Tien M (1989) Characterization of two lignin peroxidase clones from Phanerochaete chrysosporium. Biochem Biophys Res Commun 162:673–680
Asada Y, Kimura Y, Kuwahara M, Tsukamoto A, Koide K, Oka A, Takanami M (1988) Cloning and sequencing of a ligninase gene from a lignin-degrading basidiomycete, Phanerochaete chrysosporium. Appl Microbiol Biotechnol 29:469–473
Ballance DJ (1986) Sequences important in gene expression in filamentous fungi. Yeast 2:229–236
de Boer HA, Zhang YZ, Collins C, Reddy CA (1987) Analysis of nucleotide sequences of two ligninase cDNAs from a white-rot filamentous fungus, Phanerochaete chrysosporium. Gene 60:93–102
Brown A, Sims PFG, Raeder U, Broda P (1988) Multiple ligninase-related genes from Phanerochaete chrysosporium. Gene 73:77–85
Bussey H (1988) Proteases and the processing of precursors to secreted proteins in yeast. Yeast 4:17–26
Dodson PJ, Evans CS, Harvey PJ, Palmer JM (1987) Production and properties of an extracellular peroxidase from Coriolus versicolor which catalyses Cα-Cβ cleavage in a lignin model compound. FEMS Microbiol Lett 42:17–22
Farrell RL, Murtagh KE, Tien M, Mozuch MD, Kirk TK (1989) Physical and enzymatic properties of lignin peroxidase isozymes from Phanerochaete chrysosporium. Enzyme Microb Technol 11:322–328
Gold MH, Kuwahara M, Chiu AA, Glenn JK (1984) Purification and characterization of an extracellular H2O2-requiring diaryl-propane oxygenase from the white rot basidiomycete, Phanerochaete chrysosporium. Arch Biochem Biophys 234:353–362
Jönsson L, Karlsson O, Lundquist K, Nyman PO (1989) Trametes versicolor ligninase: isozyme sequence homology and substrate specificity. FEBS 247:143–146
Kantelinen A, Waldner R, Niku-Paavola M-L, Leisola MSA (1988) Comparison of two lignin-degrading fungi: Phlebia radiata and Phanerochaete chrysosporium. Appl Microbiol Biotechnol 28:193–198
Kimura Y, Asada Y, Kuwahara M (1990) Screening of basidiomycetes for lignin peroxidase genes using a DNA probe. Appl Microbiol Biotechnol 32:436–442
Kirk TK, Kersten PJ, Mozuch MD, Kalyanaraman B (1986) Ligninase of Phanerochaete chrysosporium. Mechanism of its degradation of the non-phenolic arylglycerol β-aryl ether substructure of lignin. Biochem J 236:279–287
Langford CJ, Gallwitz D (1983) Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase transcripts. Cell 33:519–527
Mizusawa S, Nishimura S, Seela F (1986) Improvement of the dideoxy chain terminator method of DNA sequencing by use of deoxy-7 deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res 14:1319–1324
Niku-Paavola M-L, Karhunen E, Salola P, Raunio V (1988) Ligninolytic enzymes of the white-rot fungus Phlebia radiata. Biochem J 254:877–884
Smith TL, Schalch H, Gaskell J, Covert S, Cullen D (1988) Nucleotide sequence of a ligninase gene from Phanerochaete chrysosporium. Nucleic Acids Res 16:1219
Tien M, Kirk TK (1984) Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proc Natl Acad Sci USA 81:2280–2284
Tien M, Tu C-PD (1987a) Cloning and sequencing of cDNA for a ligninase from Phanerochaete chrysosporium. Nature 326:520–523
Tien M, Tu C-PD (1987b) Cloning and sequencing of a cDNA for a ligninase from Phanerochaete chrysosporium. Nature 328:742
Yelton MH, Hamer JE, Timberlake WE (1984) Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci USA 81:1470–1474
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Kimura, Y., Asada, Y., Oka, T. et al. Molecular analysis of a Bjerkandera adusta lignin peroxidase gene. Appl Microbiol Biotechnol 35, 510–514 (1991). https://doi.org/10.1007/BF00169758
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DOI: https://doi.org/10.1007/BF00169758