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Molecular characterization of laccase genes from the basidiomycete Trametes hirsuta Bm-2 and analysis of the 5′ untranslated region (5′UTR)

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Abstract

The aim of this study was to identify and characterize laccase genes produced by Trametes hirsuta Bm-2 in a liquid medium, both with and without induction. The amplification of 5′and 3′regions of laccase sequences was obtained by the RACE-PCR method, and these were assembled to obtain a cDNA of total length. Two new laccase genes were isolated from basal medium (lac-B) and lignocellulosic grapefruit substrate (lac-T), both encoding open reading frames of 2566 bp. Both laccase-predicted proteins consisted of 521 amino acids, four copper-binding regions, a signal peptide, and five potential glycosilation sites (Asn-Xaa-Ser/Tre). Moreover, the deduced amino acid sequences share about 76–85% identity with other laccases of WRF. Sequence comparison showed 47 synonymous point mutations between lac-B and lac-T. In addition, 5′ untranslated regions (UTR) of laccase genes lac-B and lac-T showed differences in length and number of regulatory elements that may affect transcriptional or translational expression of these genes.

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Acknowledgement

The authors wish to express their gratitude to National Science and Technology Council, Mexico (CONACYT) for providing the financial support for this research (Project No. 248295).

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Authors and Affiliations

  1. Depto. de Ingeniería Química y Bioquímica, Tecnológico Nacional de México, Instituto Tecnológico de Mérida, Av. Tecnológico Km 4.5 S/N, 97118, Mérida, Yucatán, Mexico

    Alejandrina Pereira-Patrón, Sara Solis-Pereira & Gabriel Lizama-Uc

  2. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, 97205, Mérida, Yucatán, Mexico

    Jorge H. Ramírez-Prado

  3. Laboratorio GeMBio, Centro de Investigación Científica de Yucatán, Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, 97205, Mérida, Yucatán, Mexico

    Daisy Pérez-Brito

  4. Unidad de Energía Renovable, Centro de Investigación Científica de Yucatán, Carretera Sierra Papacal-Chuburná Puerto Km 5, 97302, Mérida, Yucatán, Mexico

    Raul Tapia-Tussell

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  1. Alejandrina Pereira-Patrón
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  2. Sara Solis-Pereira
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  3. Gabriel Lizama-Uc
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  4. Jorge H. Ramírez-Prado
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  5. Daisy Pérez-Brito
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  6. Raul Tapia-Tussell
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Contributions

All the authors contributed to this work. Tapia-Tussell and Solis-Pereira conceived, designed and wrote the paper; Pereira-Patron performed the experiments and analyzed the data; Lizama-Uc, Perez-Brito and Ramirez-Prado participated in the data analysis of untranslated region and writing of the paper. All authors reviewed and approved the manuscript.

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Correspondence to Raul Tapia-Tussell.

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Pereira-Patrón, A., Solis-Pereira, S., Lizama-Uc, G. et al. Molecular characterization of laccase genes from the basidiomycete Trametes hirsuta Bm-2 and analysis of the 5′ untranslated region (5′UTR). 3 Biotech 9, 160 (2019). https://doi.org/10.1007/s13205-019-1691-y

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