Summary
The influence of shear forces on adherent mammalian cells was investigated by means of a developed flow chamber. The viability of the cells decreased with increasing exposure level and duration. Additional, changes in the morphology of the cells due to the shear forces were observed.
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References
Feder J, Tolbert WR (1983) The large-scale cultivation of mammalian cells. Sci Am 248:36–43
Krüger JW, Young DF, Cholvin NR (1971) An in vitro study of flow response by cells. J Biomech 4:31–36
Levesque MJ, Nerem R (1985) The elongation and orientation of cultured endothelial cells in response to shear stress. J Biomech Eng 27:1021–1026
Smith CG, Greenfield PF, Randerson D (1987) Shear sensitivity of three hybridoma cell lines in suspension culture. In: Spier RE, Griffith JB (eds) Modern approaches to animal cell technology. Butterworth, Kent, UK
Stathopoulos NA, Hellums JD (1985) Shear stress effects on human embryonic kidney cells in vitro. Biotechnol Bioeng 27:1021–1026
Tramper J, Vlak J (1988) Bioreactor design for growth of shear-sensitive mammalian and insect cells. In: Upstream processes: equipment and techniques. Liss, New York, pp 199–288
White FM (1974) Viscous fluid flows. McGraw-Hill, New YOrk
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Kretzmer, G., Schügerl, K. Response of mammalian cells to shear stress. Appl Microbiol Biotechnol 34, 613–616 (1991). https://doi.org/10.1007/BF00167909
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DOI: https://doi.org/10.1007/BF00167909