Summary
Gas membrane sensors, connected to a gas chromatography, have been tested in fermentation on complex media of industrial interest. Selectivity, sensitivity and reliability have been assessed: the calibration on fermentation medium is recommended. The use of such a sensor is demonstrated in the study of the kinetics of batch and fed-batch alcoholic fermentations on beet molasses. The kinetics of acetaldehyde are especially pointed out in relation to ethanol, fusel alcohols and carbon dioxide production.
Similar content being viewed by others
References
Axelsson JP, Mandenius CF, Holst O, Hagander P, Mattiasson B (1988) Experience in using an ethanol sensor to control molasses feed-rates in bakers yeast production. Bioproc Eng 3:1–9
Lee JH, Woodward JC, Pagan RJ, Rogers PL (1981) Evaluation of semiconductor gas sensor system for on-line ethanol estimation. Biotechnol Lett 3:251–256
Maiorella BL, Wilke CL, Blanch HW (1984) Ethanol volatility in fermentation systems. Salt and metabolite effects. Ind Eng Chem Fundam 23:332–337
Phillips DH, Johnson MJ (1961) Measurement of dissolved oxygen in fermentation. Biochem Microbiol Technol 3:261–275
Pons MN, Ducouret P, Engasser JM (1986) Mass transfer characteristics of hydrophobic tubing membrane sensors. Biotechnol Lett 8:407–410
Suomalainen H, Nykänen L (1972) Formation of aroma compounds in alcoholic beverages. Kalerstein Commun 35:185–200
Webb AD, Ingraham JL (1963) Fusel oil. Adv Appl Microbiol 5:317–353
Yamané T, Matsuda M, Sada E (1981) Application of porous teflon tubing method to automatic fed-batch culture of microorganisms I. Mass transfer through porous teflon tubing. Biotechnol Bioeng 23:2483–2507
Yano T, Kobayashi T, Shimizu S (1978) Silicone tubing sensor for detection of methanol. J Ferment Technol 56:421–427
Author information
Authors and Affiliations
Additional information
Offprint requests to: M.-N. Pons
Rights and permissions
About this article
Cite this article
Groboillot, A., Pons, MN. & Engasser, JM. Monitoring of volatiles in alcoholic fermentations on molasses via a gas membrane sensor. Appl Microbiol Biotechnol 32, 37–44 (1989). https://doi.org/10.1007/BF00164820
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00164820