Skip to main content
SpringerLink
Log in

Immunohistologic diagnosis of systemic mycoses: An update

  • Published:
European Journal of Epidemiology Aims and scope Submit manuscript

Abstract

Fluorescent antibody, immunoperoxidase and gold-silver staining methods for the rapid and accurate diagnosis of systemic mycotic infections are currently performed in a few specialized laboratories. These methods have proved applicable to formalin-fixed, paraffin-embedded tissues, and are reliable for identifying therein antigens of infectious dimorphic, monomorphic filamentous, and yeast-like fungal pathogens, i.e., Aspergillus spp., Blastomyces dermatitidis, Candida spp., Coccidioides immitis, Cryptococcus neoformans, Fusarium spp., Histoplasma capsulatum, Paracoccidioides brasiliensis, Pseudallescheria boydii, and Sporothrix schenckii. Most of the available reagents are derived from multiple adsorbed polyclonal antisera. However, problems occur in the production of uniform and standardized species- or genus- specific antibodies. Monoclonal antibodies, although promising, have to date not eliminated these problems. Immunohistologic methods will become more routinely used in clinical laboratories as these problems are resolved and more sensitive and specific reagents become commercially available.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. AlbericiF., PatiesC.T., LombardiG., AjelloL., KaufmanL. and ChandlerF. (1989): Sporothrix schenckii var. luriei as the cause of sporotrichosis in Italy - Eur. J. Epidemiol. 5: 173–177.

    Google Scholar 

  2. Arrese-EstradaJ.A., StynenD., GorisA. and Pierard G.E. (1990): Identification immunohistochimique des Aspergillus par Panticorps monoclonal EB-Al. -Pathol. 10: 198–200.

    Google Scholar 

  3. CailliezJ.C., BoudrissaA., MacKenzieD.W.R. and PoulainD. (1990): Evaluation of a gold-silver staining method for detection and identification of Candida species by light microscopy - Eur. J. Clin. Microbiol. Infect. Dis. 9: 886–891.

    Google Scholar 

  4. GordonM.A., ElliottJ.C. and HawkinsT.W. (1967): Identification of Candida albicans, other Candida species and Torulopsis glabrata by means of immunofluorescence - Sabouraudia 5: 323–328.

    Google Scholar 

  5. GraybillJ.R., PatinoM. and AhrensJ. (1988): In situ localization of antigens of Histoplasma capsulatum using colloidal gold immune electron microscopy -Mycopathologia 104: 181–188.

    Google Scholar 

  6. GreenJ.H., HarrellW.K., JohnsonJ. and BensonR. (1979): Preparation of reference antisera for laboratory diagnosis of blastomycosis - J. Clin. Microbiol. 10: 1–7.

    Google Scholar 

  7. Green J.H., Harrell W.K. and Aloisio C. (1982): The preparation of monoclonal antibodies for use as control reagents in the serological diagnosis of blastomycosis. Abstract F 67, pg. 337, Abstracts of the Annual Meeting of the American Society for Microbiology.

  8. HopwoodY., PoulainD., FortierB., EvansG. and YernesA. (1986): A monoclonal antibody to a cell wall component of Candida albicans - Infect. Immun. 54: 222–227.

    Google Scholar 

  9. JacksonJ.A., KaplanW., KaufmanL. and StandardP. (1983): Development of fluorescent-antibody reagents for demonstration of Pseudallescheria boydii in tissues - J. Clin. Microbiol. 18: 668–673.

    Google Scholar 

  10. KaplanW. (1985): Identification of fungi in tissues using fluorescent antibody techniques - Clin. Immunol. News L. 6: 29–32.

    Google Scholar 

  11. KaplanW., BraggS.L., CraneS. and AhearnD.G. (1981): Serotyping Cryptococcus neoformans by immunofluorescence - J. Clin. Microbiol. 14: 313–317.

    Google Scholar 

  12. KaplanW. and ChordM.K. (1964): Production of fluorescent antibody reagents specific for the tissue form of Coccidioides immitis - Am. Rev. Respir. Dis. 89: 651–658.

    Google Scholar 

  13. KaplanW. and IvensM.S. (1960): Fluorescent antibody staining of Sporotrichum schenckii in cultures and clinical materials - J. Invest. Derm. 35:151–159.

    Google Scholar 

  14. KaplanW. and KaufmanL. (1963): Specific fluorescent antiglobulins for the detection and identification of Blastomyces dermatididis yeast-phase cells - Mycopathol. Mycol. Appl. 19: 173–180.

    Google Scholar 

  15. KaplanW. and KraftD.E. (1969): Demonstration of pathogenic fungi in formalin-fixed tissues by imunofluorescence - Am. J. Clin. Pathol. 52:420–432.

    Google Scholar 

  16. KaplanW. and Gonzales-OchoaA. (1986): Application of the fluorescent antibody technique to the rapid diagnosis of sporotrichosis - J. Lab. Clin. Med. 62: 835–841.

    Google Scholar 

  17. KaufmanL. (1992): Laboratory methods for the diagnosis and confirmation of systemic mycoses -Clin. Infect. Dis. 14 (Suppl. 1): S23–29.

    Google Scholar 

  18. KaufmanL. and BlumerS. (1986): Occurrence of serotypes among Histoplasma capsulatum strains - J. Bacteriol. 91: 1434–1439.

    Google Scholar 

  19. KaufmanL. and BlumerS. (1968): Development and use of a polyvalent conjugate to differentiate Histoplasma duboisii from other pathogens - J. Bacteriol. 95: 1243–1246.

    Google Scholar 

  20. KleinB.S. and JonesJ.M. (1990): Isolation, purification and radiolabeling of a novel 120 kD surface protein on Blastomyces dermatitidis yeasts to detect antibody in infected patients - J. Clin. Invest. 85: 152–161.

    Google Scholar 

  21. OkudaC., ItoM., SatoY., OkaK. and HotchiM. (1987): Disseminated cutaneous Fusarium infection with vascular invasion in a leukemic patient - J. Med. Vet. Mycol. 25: 177–186.

    Google Scholar 

  22. PhillipsP. and WeinerM.H. (1987): Invasive aspergillosis diagnosed by immunohistochemistry with monoclonal and polyclonal reagents - Hum. Pathol. 18: 1015–1024.

    Google Scholar 

  23. PidcoeV. and KaufmanL. (1968): Fluorescent-antibody reagent for the identification of Cryptococcus neoformans - Appl. Microbiol. 16: 271–275.

    Google Scholar 

  24. RusselB., BeckettJ.H. and JacobsP.H. (1979): Immunoperoxidase localization of Sporothrix schenckii and Cryptococcus neoformans - Arch. Dermatol. 115: 433–435.

    Google Scholar 

  25. SilvaM.E. and KaplanW. (1985): Specific fluorescein-labeled antiglobulins for the yeast form of Paracoccidioides brasiliensis - Am. J. Trop. Med. Hyg. 14: 290–294.

    Google Scholar 

  26. WattsJ.C., ChandlerF.W., MilhalovM.L., KammeyerP.L. and ArminA.R. (1990): Giant forms of Blastomyces dermatitidis in the pulmonary lesions of blastomycosis. Potential confusion with Coccidioides immitis - Am. J. Clin. Pathol. 93: 575–578.

    Google Scholar 

  27. WeissenbockH., LoupalG., KaufmanL. and KuttinE.S. (1990): phaohyphomykose-2 Fall berichte bei hund und katzeund eine literatura bersicht - Wein-Tieparzt Mschr. 77: 277–284.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Mycotic Diseases Branch - Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases - Centers for Disease Control, Mailstop G-11, 30333, Atlanta, Georgia

    L. Kaufman

Authors
  1. L. Kaufman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaufman, L. Immunohistologic diagnosis of systemic mycoses: An update. Eur J Epidemiol 8, 377–382 (1992). https://doi.org/10.1007/BF00158571

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00158571

Key words

Search

Navigation