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Infectious Diseases

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Handbook of Practical Immunohistochemistry

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Abstract

Detection and identification of infectious microorganisms involves the use of conventional immunohistochemistry in addition to many other techniques including culture, serology, histochemistry, in situ hybridization, polymerase chain reaction and direct fluorescence antibody assays. This updated chapter takes into consideration all of these techniques while answering questions about bacterial, mycobacterial viral, fungal and protozoan testing. The best techniques and testing conditions are described for dozens of the most clinically relevant microorganisms. The role of immunohistochemistry versus alternative techniques is clearly presented. Photomicrographs present the characteristic feature of optimized staining techniques. Topics for each organism are discussed including the sensitivity and specificity of the tests, how fixation and retrieval affect the results, when protease should be considered in an assay, and how these tests could be incorporated into your clinical practice.

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Authors and Affiliations

  1. Ackerman Academy of Dermatopathology, 145 E. 32nd St., 10th Floor, New York, NY, 10016, USA

    Dirk M. Elston MD

  2. Dermatology and Laboratory Medicine, Pathology, Mayo Clinic, Rochester, MN, USA

    Lawrence E. Gibson MD

  3. Dermatopathology Friedrichshafen, Friedrichshafen, Germany

    Heinz Kutzner

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  1. Dirk M. Elston MD
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  2. Lawrence E. Gibson MD
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  3. Heinz Kutzner
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Correspondence to Dirk M. Elston MD .

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Editors and Affiliations

  1. Laboratory Medicine, Geisinger Health System, Danville, Pennsylvania, USA

    Fan Lin

  2. Laboratory Medicine, Geisinger Medical Center, Danville, Pennsylvania, USA

    Jeffrey Prichard

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Elston, D.M., Gibson, L.E., Kutzner, H. (2015). Infectious Diseases. In: Lin, F., Prichard, J. (eds) Handbook of Practical Immunohistochemistry. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1578-1_32

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