Abstract
Pulsed field gel electrophoresis was used for differentiation of Coxiella burnetii isolates derived from animals in Europe and one from Namibia. Previously published data on isolates from North America demonstrated four groups with distinct banding patterns. Fingerprints of prototype isolates as references confirmed four different cleavage patterns upon NotI restriction of total DNA as described. With isolates available at our institute five additional DNA banding patterns were obtained from European and one Namibian isolate after endonuclease restriction with Notl. To compare patterns from different electrophoretic runs more precisely an image analysis system was used.
Article PDF
Similar content being viewed by others
References
Frazier ME., Mallavia L.P., Samuel J.E. and Baca O.G. (1990): DNA probes for the identification of Coxiella burnetii strains. In Rickettsiology: Current Issues and Perspectives, eds. Hechemy K.E., Paretsky D., Walker D.H. and Mallavia L.P. — Ann. N.Y. Acad. Sci. 590: 445–456.
Hackstadt T. (1986): Antigenic variation in phase I lipopolysaccharide of Coxiella burnetii isolates —Infect. Immun. 52: 337–340.
Heinzen R., Stiegler G.L., Whiting L.L., Schmitt S.A., Mallavia L.P. and Frazier M.E. (1990): Use of pulsed field gel electrophoresis to differentiate Coxiella burnetii strains. In Rickettsiology: Current Issues and Perspectives, eds. Hechemy K.E., Paretsky D., Walker D.H. and Mallavia L.P. - Ann. N.Y. Acad. Sci. 590: 504–513.
Hendrix L.R., Samuel J.E. and Mallavia L.P. (1991): Differentiation of Coxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE - J. Gen. Microbiol. 137: 269–276.
Mallavia L.P. and Samuel J.E. (1989): Genetic diversity of Coxiella burnetii. In: Intracellular Parasitism, ed. James Moulder - CRC Press, Boca Raton, Fla., pp. 117–126.
Sambrook J., Fritsch E.F. and Maniatis T. (1989): In Molecular cloning. A laboratory manual, second edition - Cold Spring Harbor Laboratory Press pp. 6–56.
Samuel J.E., Frazier M.E. and Mallavia L.P. (1985): Correlation of plasmid type and disease caused by Coxiella burnetii - Infect. Immun. 49: 775–779.
Trodkin M.H., Williams J.C. and Stephenson E.H. (1986): Genetic heterogeneity among isolates of Coxiella burnetii - J. Gen. Microbiol. 132: 455–463.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thiele, D., Willems, H., Köpf, G. et al. Polymorphism in DNA restriction patterns of Coxiella burnetii isolates investigated by pulsed field gel electrophoresis and image analysis. Eur J Epidemiol 9, 419–425 (1993). https://doi.org/10.1007/BF00157400
Issue Date:
DOI: https://doi.org/10.1007/BF00157400