Skip to main content
Log in

High ethanol production by new isolates of Clostridium thermocellum

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Among twelve strains of Clostridium thermocellum isolated from faecal droppings of various herbivorous animals and birds, three of the strains, SS21, SS22 and SS19, produced 0.37, 0.33 and 0.32 g of ethanol per g of the substrate consumed and had ethanol to acetate ratios of 2.21, 2.45 and 1.72 respectively. These are the highest substrate conversion yields of ethanol amongst the wild strains of C. thermocellum reported so far. The optimum temperature and pH for growth and ethanol production were 60 °C and 7.5, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barker, S.B. and Summerson, W.H. (1941). J.Biol. Chem. 138, 535–554.

    Google Scholar 

  • Bender, J., Vatcharapijarn, Y. and Jeffries, T.W.(1985). Appl. Environ. Microbiol. 49, 475–477.

    Google Scholar 

  • Freier, D., Mothershed, C.P. and Wiegel, J. (1988). Appl. Environ. Microbiol. 49, 204–211.

    Google Scholar 

  • Johnson, E.A., Madia, A. and Demain, A.L. (1981). Appl. Environ. Microbiol. 41, 1060–1062.

    Google Scholar 

  • Lamed, R.J., Lobos, J.H. and Su, T.M. (1988). Appl. Environ. Microbiol. 54, 1216–1221.

    Google Scholar 

  • Lee, B.H. and Blackburn, T.H. (1975). Appl. Microbiol. 30, 346–353.

    Google Scholar 

  • Lovitt, R.W., Kim, B.H., Shen, G.J. and Zeikus, J.G. (1988). CRC Crit. Rev. Biotechnol. 7, 107–186.

    Google Scholar 

  • Lynd, L.R. (1989). Adv. Biochem. Engg/Biotechnol. 38, 2–52.

    Google Scholar 

  • McBee, R.H. (1950). Bacteriol. Rev. 14, 51–63.

    Google Scholar 

  • Mori, Y. (1990). Appl. Environ. Microbiol. 56, 37–42.

    Google Scholar 

  • Ng. T.K., Ben-Bassat, A. and Zeikus, J.G. (1981). Appl. Environ. Microbiol. 41, 1337–1343.

    Google Scholar 

  • Sai Ram, M. and Seenayya, G. (1989). Biotechnol. Letts. 11,589–592.

    Google Scholar 

  • Sai Ram, M., Swamy, M.V.and Seenayya, G.(1991). Indian J. Microbiol.31, 175–180.

    Google Scholar 

  • Sato, K., Goto, S., Yonemura, S., Sekine, K., Okuma, E., Takagi, Y., Hon-Nami, K. and Saiki, T. (1992). Appl. Environ. Microbiol. 58, 734–736.

    Google Scholar 

  • Slapack, G.E., Russell, I., and Stewart, G.G. (1985). NRCC No.24410 Project Report submitted to Division of Energy, NRC, Ottawa, 404.

    Google Scholar 

  • Sudha Rani, K., Swamy, M.V., Sunitha, D. and Seenayya, G.(1994), Biotechnol Letts. 16, 201–204.

    Google Scholar 

  • Tailliez, P., Girard, H., Longin, R., Beguin, P. and Millet, T. (1989). Appl. Environ. Microbiol. 55, 203–206.

    Google Scholar 

  • Tailliez, P., Girard, H., Millet, J. and Beguin, P. (1989). Appl. Environ. Microbiol. 55, 207–211.

    Google Scholar 

  • Weimer, P.J. and Zeikus, J.G. (1977). Appl. Environ. Microbiol. 33, 289–297.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rani Sudha, K., Swamy, M.V. & Seenayya, G. High ethanol production by new isolates of Clostridium thermocellum . Biotechnol Lett 18, 957–962 (1996). https://doi.org/10.1007/BF00154629

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00154629

Keywords

Navigation