Summary
A rapid assay (response time of the order of minutes) using membrane inlet mass spectrometry was employed to assess the immediate effects of chemicals on methanogenesis. Changes in the levels of CH4, H2, O2 and H2S were continuously and simultaneously monitored directly in the liquid phase, in response to the presence of oxygen, sodium dodecylbenzenesulfonate, chlorhexidine diacetate, molybdate, sulphate, bromoethanesulfonate, formate, propionate or isobutyric acid.
Similar content being viewed by others
References
Abram, J.W. and Nedwell, D.B. (1978). Arch. Microbiol. 117, 89–92.
Benstead, J., Archer, D.B. and Lloyd, D. (1990). Role of Hydrogen in the Growth of Mutualistic Methanogenic Cocultures. In: Microbiology and Biochemistry of Strict Anaerobes involved in Interspecies Hydrogen Transfer. J-P Belaich, M Bruschi and J-L Garcia eds., pp 161–171 New York: Plenum Press
Benstead, J., Archer, D.B. and Lloyd, D. (1991). Arch. Microbiol. 156, 34–37.
Bohátka, S., Langer, G., Szilágyi, J., and Berecz, I. (1983). Int. J. Mass Spectrom. Ion Phys. 48, 277–280
Ferguson, T.J. and Mah, R.A. (1983). Appl. Environ. Microbiol. 46, 348–355.
Khalil, E.F., Whitmore, T.N., Gamal-El-Din, H., El-Bassel, A. and Lloyd, D. (1988). Appl. Microbiol. Biotechnol. 29, 517–522.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). J. Biol. Chem. 193, 265–275.
Mosey, F.E.(1982). Water Pollut. Cont. 81, 540–552.
Postgate, J.R. (1984). The sulphate-reducing bacteria. 2nd edn., Cambridge:Cambridge University Press
Smith, M.R. (1983). J. Bacteriol. 156, 516–523.
Tatton, M.J.,Archer, D.B., Powell, G.E. and Parker, M.L.(1989). Appl. Environ. Microbiol. 55, 440–445.
Taylor, B.F. and Oremland, R.S. (1979).Curr. Microbiol. 3, 101–103.
Whitmore, T.N., Lazzari, M. and Lloyd, D. (1985). Biotechnol. Lett. 7, 283–288.
Whitmore, T.N. and Lloyd, D. (1986). Biotechnol. Lett. 8, 203–208.
Whitmore, T.N., Lloyd, D., Jones, G. and Williams, T.N. (1987). Appl. Microbiol. Biotechnol. 26, 383–388.
Wilhelm, E., Battino, R. and Wilcock, R.J. (1977). Chem. Rev. 77, 219–262.
Winfrey, M.R. and Zeikus, J.G. (1977). Appl. Environ. Microbiol. 33, 275–281.
Yadav, V.K. and Archer, D.B.(1988). Lett. Appl. Microbiol. 7, 165–168.
Zinder, S.H. (1984). ASM News 50, 294–298.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Benstead, J., Archer, D.B. & Lloyd, D. Rapid method for monitoring methanogenic activities in mixed culture: Effects of inhibitory compounds. Biotechnol Tech 7, 31–36 (1993). https://doi.org/10.1007/BF00151086
Issue Date:
DOI: https://doi.org/10.1007/BF00151086