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Rapid method for monitoring methanogenic activities in mixed culture: Effects of inhibitory compounds

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Biotechnology Techniques

Summary

A rapid assay (response time of the order of minutes) using membrane inlet mass spectrometry was employed to assess the immediate effects of chemicals on methanogenesis. Changes in the levels of CH4, H2, O2 and H2S were continuously and simultaneously monitored directly in the liquid phase, in response to the presence of oxygen, sodium dodecylbenzenesulfonate, chlorhexidine diacetate, molybdate, sulphate, bromoethanesulfonate, formate, propionate or isobutyric acid.

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References

  • Abram, J.W. and Nedwell, D.B. (1978). Arch. Microbiol. 117, 89–92.

    Google Scholar 

  • Benstead, J., Archer, D.B. and Lloyd, D. (1990). Role of Hydrogen in the Growth of Mutualistic Methanogenic Cocultures. In: Microbiology and Biochemistry of Strict Anaerobes involved in Interspecies Hydrogen Transfer. J-P Belaich, M Bruschi and J-L Garcia eds., pp 161–171 New York: Plenum Press

  • Benstead, J., Archer, D.B. and Lloyd, D. (1991). Arch. Microbiol. 156, 34–37.

    Google Scholar 

  • Bohátka, S., Langer, G., Szilágyi, J., and Berecz, I. (1983). Int. J. Mass Spectrom. Ion Phys. 48, 277–280

    Google Scholar 

  • Ferguson, T.J. and Mah, R.A. (1983). Appl. Environ. Microbiol. 46, 348–355.

    Google Scholar 

  • Khalil, E.F., Whitmore, T.N., Gamal-El-Din, H., El-Bassel, A. and Lloyd, D. (1988). Appl. Microbiol. Biotechnol. 29, 517–522.

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). J. Biol. Chem. 193, 265–275.

    Google Scholar 

  • Mosey, F.E.(1982). Water Pollut. Cont. 81, 540–552.

    Google Scholar 

  • Postgate, J.R. (1984). The sulphate-reducing bacteria. 2nd edn., Cambridge:Cambridge University Press

    Google Scholar 

  • Smith, M.R. (1983). J. Bacteriol. 156, 516–523.

    Google Scholar 

  • Tatton, M.J.,Archer, D.B., Powell, G.E. and Parker, M.L.(1989). Appl. Environ. Microbiol. 55, 440–445.

    Google Scholar 

  • Taylor, B.F. and Oremland, R.S. (1979).Curr. Microbiol. 3, 101–103.

    Google Scholar 

  • Whitmore, T.N., Lazzari, M. and Lloyd, D. (1985). Biotechnol. Lett. 7, 283–288.

    Google Scholar 

  • Whitmore, T.N. and Lloyd, D. (1986). Biotechnol. Lett. 8, 203–208.

    Google Scholar 

  • Whitmore, T.N., Lloyd, D., Jones, G. and Williams, T.N. (1987). Appl. Microbiol. Biotechnol. 26, 383–388.

    Google Scholar 

  • Wilhelm, E., Battino, R. and Wilcock, R.J. (1977). Chem. Rev. 77, 219–262.

    Google Scholar 

  • Winfrey, M.R. and Zeikus, J.G. (1977). Appl. Environ. Microbiol. 33, 275–281.

    Google Scholar 

  • Yadav, V.K. and Archer, D.B.(1988). Lett. Appl. Microbiol. 7, 165–168.

    Google Scholar 

  • Zinder, S.H. (1984). ASM News 50, 294–298.

    Google Scholar 

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Benstead, J., Archer, D.B. & Lloyd, D. Rapid method for monitoring methanogenic activities in mixed culture: Effects of inhibitory compounds. Biotechnol Tech 7, 31–36 (1993). https://doi.org/10.1007/BF00151086

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