Skip to main content
Log in

Starch hydrolysis using enzyme in supercritical carbon dioxide

  • Published:
Biotechnology Techniques

Summary

The enzymatic hydrolysis reaction with supercritical carbon dioxide(SC-CO2) as a reaction medium to make glucose from starch was investigated. The reaction rate was enhanced at higher temperature and pressure, especially near the critical point of the CO2. The α-amylase and glucoamylase were found to be active in a SC-CO2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Chi, Y. M., Nakamura, K., and Yano, T. (1988) Enzymatic interesterification in supercritical carbon dioxide. Agric. Biol. Chem., 52, 1541–1550.

    Google Scholar 

  • Lee, H., Chung, B. H., and Park, Y. H. (1991) Concentration of Tocopherols from Soybean sludge by supercritical carbon dioxide. J. Am. Oil Chem. Soc., 68, 571–573.

    Google Scholar 

  • Miller, D. A., Blanch, H. W., and Praunitz, J. M. (1991) Enzyme-catalyzed interesterification of triglycerides in supercritical carbon dioxide. Ind. Eng. Chem. Res., 30, 939–946.

    Google Scholar 

  • Miller, G. L. (1959) Use of dinitrosalicyclic acid reagent for determination of reduceing sugar. Anal. Chem., 31, 426.

    Google Scholar 

  • Nakamura, K. (1990) Biochemical reactions in supercritical fluids. Trends in Biotechnol., 8, 288–292.

    Google Scholar 

  • Pasta, P., Mazzola, G., Carrea, G., and Riva, S. (1989) Subtilisin-catalyzed transesterification in supercritical carbon dioxide. Biotechnol. Lett., 9, 643–648.

    Google Scholar 

  • Subramanian, B. and McHugh, M. A. (1986) Reactions in supercritical fluids- a review. Ind. Eng. Chem. Process Des., 25, 1–12.

    Google Scholar 

  • Vick Roy, J. R., and Converse, A. O. (1985) Biomass Hydrolysis with sulfur dioxide and water in the region of the critical point. In Supercritical Fluid Technology, edited by J. M. L. Penninger, M. Radosz, M. A. McHugh and V. J. Krukonis, 1st edn, pp. 397–414, Amsterdam: Elsevier Science Publishers.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, HS., Lee, W.G., Park, SW. et al. Starch hydrolysis using enzyme in supercritical carbon dioxide. Biotechnol Tech 7, 267–270 (1993). https://doi.org/10.1007/BF00150896

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00150896

Keywords

Navigation