Abstract
Direct batch addition of sterile Chelex ion-exchange resin to Dubecco's modified Eagle's medium supplemented with 10% fetal calf serum with gentle stirring removed a very wide variety of trace metal ions from the medium to varying extents dependent upon Chelex content (between 0.01 and 4% w/v), exposure time (between 5 min and 10 days) and temperature 4, 25 and 37 °C). Prolonged treatment (10 days) with 4% w/v Chelex at 4°C reduced the concentration of zinc, strontium, aluminum, copper, manganese, nickel and chromium from 100 to 2.7, 12.1, 7.7, 22.6, 13.0, 14.7 and 53.3% of their original concentrations, respectively. Re-supplementation of the metal depleted medium with a defined cocktail of metals restored the growth potential of the medium which was then capable of supporting growth over at least three subcultures without a decrease in fibroblast cell yield, demonstrating its suitability in cell culture studies on trace metal ions.
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Reference
Rayner MH, Suzuki KT. 1994 Effect of medium copper concentration on the growth, uptake and intracellular balance of copper and zinc in Menkes and normal fibroblast cells. BioMetals 7, 253–260.
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Rayner, M.H., Suzuki, K.T. A simple and effective method for the removal of trace metal cations from a mammalian culture medium supplemented with 10% fetal calf serum. Biometals 8, 188–192 (1995). https://doi.org/10.1007/BF00143374
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DOI: https://doi.org/10.1007/BF00143374