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Inulin hydrolysis by the Debaryomyces phaffii inulinase immobilized on Deae cellulose

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Abstract

Debaryomyces phaffii inulinase was immobilized by adsorption on DEAE-cellulose. The immobilization caused a drop in optimum pH, a slight increase in optimum temperature and an important increase in the thermal stability of the system. The activity yield of immobilized inulinase was 75%.

A continuous reactor operation was carried out. The utilization of this system permited the production of fructose syrup from inulin.

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Authors and Affiliations

  1. Sciences et Technologies des Industries Alimentaires USTL, 34060, Montpellier, France

    Joseph Pierre Guiraud, Sylvie Demeulle & P. Galzy

  2. Laboratoire de Génétique et Microbiologie ENSAM/INRA, 34060, Montpellier, France

    P. Galzy

Authors
  1. Joseph Pierre Guiraud
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  2. Sylvie Demeulle
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  3. P. Galzy
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Guiraud, J.P., Demeulle, S. & Galzy, P. Inulin hydrolysis by the Debaryomyces phaffii inulinase immobilized on Deae cellulose. Biotechnol Lett 3, 683–688 (1981). https://doi.org/10.1007/BF00134844

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