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Secretion and localization of invertase and inulinase in recombinant Saccharomyces cerevisiae

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Summary

Secretion of invertase and inulinase produced by recombinant Saccharomyces cerevisiae cells were investigated under derepression conditions of GALI promoter. Secreted invertase mainly localized in the periplasmic space, but most of inulinase was found in the extracellular culture medium. This high level of extracellular secretion of inulinase was not dependent on the growth phase in which derepression of GALI promoter occurs. Our results indicate that the inulinase polypeptide itself may have a function for the protein secretion into the culture medium.

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Authors and Affiliations

  1. Genetic Engineering Research Institute, KIST, P.O. Box 17, Taejon, Korea

    Soo-Wan Nam

  2. Dept. of Agricultural Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113, Tokyo, Japan

    Koji Yoda & Makari Yamasaki

Authors
  1. Soo-Wan Nam
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  2. Koji Yoda
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  3. Makari Yamasaki
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Nam, SW., Yoda, K. & Yamasaki, M. Secretion and localization of invertase and inulinase in recombinant Saccharomyces cerevisiae . Biotechnol Lett 15, 1049–1054 (1993). https://doi.org/10.1007/BF00129936

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