Skip to main content
Log in

Effect of pre-immobilization conditions on phosphate uptake by immobilized Chlorella

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Batch culture studies of phosphate uptake by non-immobilized (free) and immobilized (Ca-alginate-entrapped) Chlorella emersonii have shown that exponentially growing free cells remove phosphate from the medium five times more rapidly than cells in late stationary phase. Culture age is also shown to be an important factor in determining the uptake abilities of cells when in their immobilized state. When cells of different ages are immobilized in Ca-alginate and placed in a small-scale packed-bed reactors the effects of culture age are sufficient to produce significant differences in reactor performance lasting in the order of five days.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Cembella, A.D., Antia, N.J., and Harrison, P.J. (1984). The utilization of inorganic and organic phosphorous compounds as nutrients by eukaryotic microalgae: A multidisciplinary perspective: Part 1. In:Critical Reviews in Microbiology, W.M. O'Leary, ed. vol 10, pp. 317–391, Boca Raton, CRC Press.

    Google Scholar 

  • de la Noüe, J., and Proulx, D. (1988). Appl. Microbiol Biotechnol. 29, 292–297.

    Google Scholar 

  • Garbisu, C., Hall, D.O., and Serra, J.L. (1993). J. Chem. Tech. Biotechnol. 57, 181–189.

    Google Scholar 

  • Golterman, H.L., Clymo, R.S., and Ohnstad, M.A.M. (1978). Methods for Physical and Chemical Analysis of Fresh Waters, Oxford, Blackwell Scientific Publications.

    Google Scholar 

  • Kierstan, M., and Bucke, C. (1977). Biotechnol. Bioeng. 19, 387–397.

    Google Scholar 

  • Megharaj, M., Pearson, H.W., and Venkateswarlu, K. (1992). Enzyme Microb. Technol. 14, 656–658.

    Google Scholar 

  • Nichols, H.W. (1973). Growth media - freshwater. In:Handbook of Phycological Methods: Culture Methods and Growth Measurements, J.R. Stein ed. pp. 7–24, Cambridge, Cambridge University Press.

    Google Scholar 

  • Robinson, P.K., Reeve, J.O., and Goulding, K.H. (1989). Enzyme Microb. Technol. 11, 590–596.

    Google Scholar 

  • Robinson, P.K., and Wilkinson, S.C. (1994). Enzyme Microb. Technol. 16, 802–807.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Robinson, P.K. Effect of pre-immobilization conditions on phosphate uptake by immobilized Chlorella . Biotechnol Lett 17, 659–662 (1995). https://doi.org/10.1007/BF00129396

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00129396

Keywords

Navigation