Skip to main content
SpringerLink
Log in

Scleroglucan and oxalic acid formation by Sclerotium glucanicum in sucrose supplemented fermentations

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Strategies for adding sucrose to stationary phase cultures of Sclerotium glucanicum to improve scleroglucan production were examined. Particular attention was paid to the effect of sucrose supplementation on the formation of the toxic by-product, oxalic acid. The rate of addition of sucrose was found to markedly influence the outcome of the process, with addition at a rate of 0.084 gl−1h−1 giving a 50% improvement in broth biopolymer concentration. Additions at higher rates seemed to cause inhibition of the culture. However, greatly increased oxalic acid concentrations in the sucrose supplemented fermentations, and the impact of unfavourable changes in productivity and specific productivity call into question the utility of this proposed method of process improvement for this microorganism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sandford, P.A. (1979). Adv. Carbohydr. Chem. Biochem. 36, 265–295.

    Google Scholar 

  2. Holzwarth, G. (1985). Dev. Ind. Microbial. 26, 271–280.

    Google Scholar 

  3. Singh, P.P., Whistler, R.L., Tokuzen, R. and Nakahara, W. (1974). Carbohydr. Res. 37, 245–247.

    Google Scholar 

  4. Pretus, H.A., Ensley, H.E., McNamee, R.B., Jones, E.L., Browder, I.W. and Williams, D.L. (1991). J. Pharmacol. Exp. Therap. 257. 500–510.

    Google Scholar 

  5. Jong, S.C. and Denovick, R. (1987). Adv. Appl. Microbiol. 34, 183–262.

    Google Scholar 

  6. Rau, U., Gura, E., Olszewski, E. and Wagner, F. (1992). J. Industrial Microbiol. 9, 19–26.

    Google Scholar 

  7. McNeil, B. and Harvey, L.M. (1993). Crit. Rev. Biotechnol. 13(4), 275–304.

    Google Scholar 

  8. Taurhesia, S. (1992). Exopolysaccharide production by submerged culture of Sclerotium glucanicum. PhD Thesis, University of Strathclyde, Glasgow, UK.

    Google Scholar 

  9. Taurhesia, S., and McNeil, B. (1994). Production of scleroglucan by Sclerotium glucanicum in batch and supplemented batch cultures. Enz. Microbial Technol. (in press).

  10. Maxwell, D.P. and Bateman, D.F. (1968). Phytopathol. 88, 1635–1639.

    Google Scholar 

  11. Punja, Z.K. and Jenkins, S.F. (1984). Mycologia 84, 467–469.

    Google Scholar 

  12. Pilz, F., Auling, G., Stephan, D., Rau, V. and Wagner, F. (1991). Exp. Mycol. 15, 181–192.

    Google Scholar 

  13. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956). Anal. Chem. 28, 350–356.

    Google Scholar 

  14. Margaritis, A. and Pace, G.W. (1985). Microbial Polysaccharides. In Comprehensive Biotechnology Vol. 3. (eds. H.W. Blanch, S. Drew and D.I.C. Wang), Pergamon Press, New York, pp. 1031–1036.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bioscience and Biotechnology, University of Strathclyde, G1 1XW, Glasgow, UK

    Y. Wang & B. McNeil

Authors
  1. Y. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. McNeil
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Y., McNeil, B. Scleroglucan and oxalic acid formation by Sclerotium glucanicum in sucrose supplemented fermentations. Biotechnol Lett 16, 605–610 (1994). https://doi.org/10.1007/BF00128608

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00128608

Keywords

Search

Navigation