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Conjugative transfer of plasmid pTO1 from Escherichia coli to Rhodococcus spp.

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Summary

We demonstrated the conjugative transfer of plasmid pTO1 from Escherichia coli S17-1 to different Rhodococcus spp. The plasmid contains the oriT fragment from RK2 and a fragment of Streptomyces φC31 actinophage with the attachment site and the integration genes. Experiments on hybridization showed that plasmid pTO1 is chromosomally integrated into the Rhodococcus cells.

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Authors and Affiliations

  1. Institute of Genetics and Selection of Industrial Microorganisms, I Dorozhny proezd 1, 113545, Moscow, Russia

    T. Voeykova, V. Tabakov, L. Ryabchenko, N. Mkrtumyan & A. Yanenko

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  1. T. Voeykova
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  2. V. Tabakov
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  3. L. Ryabchenko
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  4. N. Mkrtumyan
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  5. A. Yanenko
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Voeykova, T., Tabakov, V., Ryabchenko, L. et al. Conjugative transfer of plasmid pTO1 from Escherichia coli to Rhodococcus spp.. Biotechnol Lett 16, 555–560 (1994). https://doi.org/10.1007/BF00128599

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