Skip to main content
SpringerLink
Log in

Hyaluronic acid secreted by mesothelial cells: a natural barrier to ovarian cancer cell adhesion

  • Published:
Clinical & Experimental Metastasis Aims and scope Submit manuscript

The adhesion to mesothelial monolayers of eight cultured ovarian tumour cell lines was studied in multiwell plates as a model for some of the interactions of ovarian cancer in the peritoneal cavity. When only the upper half of the conditioned medium (CM) from a confluent mesothelial cell culture was aspirated, the adhesion of the tumour cells was low (3.5%–36%). When the medium was removed completely the adhesion increased. The tumour cell lines showing the greatest enhancement of adhesion were those which had previously been shown to express the highest amounts of CD44. By adding erythrocyte suspensions to mesothelial cells it was shown that there was a pericellular coat around the mesothelial cells that could be destroyed by aspirating the medium, or by treating the medium with hyaluronidase (Hase). Treatment of the CM with Hase also considerably increased tumour cell adhesion. Furthermore, CM was shown to contain high amounts of hyaluronic acid (HA). HA blocked adhesion in the absence of CM, but the effect was not as large as that produced by the pericellular coat. It is proposed that pericellular HA produced by mesothelial cells has an important role in the invasion of ovarian tumour cells in the peritoneal cavity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Office of Population Censuses and Surveys, 1994, Cancer Statistics, 1989, HMSO.

  2. Hoskins WJ, 1993, Surgical scraping and cytoreductive surgery of epithelial ovarian cancer. Cancer, 71, 1534–40.

    Google Scholar 

  3. Vracko RV, 1974, Basal lamina scaffold anatomy and significance for the maintenance of orderly tissue structure. Am J Pathol, 77, 314–46.

    Google Scholar 

  4. Catterall JB, Gardner MJ, Jones LMH, Thompson G and Turner GA, 1994, A precise, rapid, and sensitive assay to measure the adhesion of ovarian tumour cells to peritoneal mesothelial cells. Cancer Lett, 87, 199–203.

    Google Scholar 

  5. Gardner MJ, Jones LMH, Catterall JB and Turner GA, 1995, Expression of cell adhesion molecules on ovarian tumour cell lines and mesothelial cells in relation to ovarian cancer metastasis. Cancer Lett, 91, 527–34.

    Google Scholar 

  6. Hills CA, Kelland LR, Abel G, Sirachy J, Wilson AP and Harrap KR, 1989, Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes. Br J Cancer, 59, 527–34.

    CAS  PubMed  Google Scholar 

  7. Mobus V, Gerharz CD, Press V, et al. 1992, Morphological, immunological, histochemical and biochemical characterization of 6 newly established human ovarian tumour carcinoma cell lines. Int J Cancer, 52, 76–84.

    Google Scholar 

  8. Heldin P and Pertoft H, 1993, Synthesis and assembly of the hyaluronan-containing coats around normal mesothelial cells. Exp Cell Res, 208, 422–9.

    Google Scholar 

  9. Niedbala MJ, Crikard K and Bernaki RJ, 1985, Interactions of human ovarian tumour cells with human mesothelial cells grown on extracellular matrix. Exp Cell Res, 160, 499–513.

    Google Scholar 

  10. Cannistra SA, Kansas GS, Niloff J, et al. 1993, Binding of human ovarian cancer cells to peritoneal mesothelium is partly mediated by CD44H. Cancer Res, 53, 3830–8.

    Google Scholar 

  11. Laurent TC and Fraser RE, 1992, Hyaluronan. FASEB, 6, 2397–404.

    Google Scholar 

  12. Clarris BJ, Fraser JRE and Rodda SJ, 1974, Effect of cell-bound hyaluronic acid on infectivity of Newcastle disease virus for human sinovial cells in vitro. Ann Rheum Dis, 33, 240–2.

    Google Scholar 

  13. McBride WM and Bard JBL, 1979, Hyaluronidasesensitive halos around adherent cells. Their role in blocking lymphocyte-mediated cytolysis. J Exp Med, 149, 507–15.

    Google Scholar 

  14. Knudson CB and Toole BP, 1985, Changes in the pericellular matrix during differentiation of limb bud mesoderm. Dev Biol, 112, 308–18.

    Google Scholar 

  15. Aruffo A, Stamenkovic I, Melnick M, et al. 1990, CD44 is the principal cell surface receptor for hyaluronate. Cell, 161, 1303–13.

    Google Scholar 

  16. Lesley J, Hyman R and Kincade PW, 1993, CD44 and its interaction with extracellular matrix. Adv Immunol, 151, 271–335.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Clinical Biochemistry, The Medical School, Newcastle upon Tyne, UK

    Lindsay M. H. Jones, Michael J. Gardner, Jonathon B. Catterall & Graham A. Turner

Authors
  1. Lindsay M. H. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael J. Gardner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jonathon B. Catterall
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Graham A. Turner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jones, L.M.H., Gardner, M.J., Catterall, J.B. et al. Hyaluronic acid secreted by mesothelial cells: a natural barrier to ovarian cancer cell adhesion. Clin Exp Metast 13, 373–380 (1995). https://doi.org/10.1007/BF00121913

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00121913

Keywords

Search

Navigation