Abstract
Mesothelial cells line the serosal cavities and associated organs. In order to metastasize to distant organs, ovarian tumor cells must first attach and then clear the mesothelial cells. Therefore, human primary mesothelial cells (HPMCs) are necessary to effectively study ovarian cancer metastases. Here, we describe methods to obtain HPMCs from human omentum and to culture in vitro.
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Mullen, M., Noia, H., Fuh, K. (2022). Culturing Primary Human Mesothelial Cells. In: Kreeger, P.K. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 2424. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1956-8_9
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DOI: https://doi.org/10.1007/978-1-0716-1956-8_9
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1955-1
Online ISBN: 978-1-0716-1956-8
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