Abstract
The interaction between gel concentration, cytokinin levels and temperature in vitrification of Olearia microdisca in vitro has been investigated. Vitrified plants displayed tissue hypertrophy and reduced lignification. Cytokinin in the medium was essential for vitrification. Increasing gel concentration or reducing temperature reduced the vitrification in the presence of 20 µM BA. The observed responses were consistent with indirect effects on BA uptake. Alternatively an indirect effect of BA on shoot multiplication rate and hence on lignification of cell walls may be involved.
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Williams, R.R., Taji, A.M. Effect of temperature, gel concentration and cytokinins on vitrification of Olearia microdisca (J.M. Black) in vitro shoot cultures. Plant Cell Tiss Organ Cult 26, 1–6 (1991). https://doi.org/10.1007/BF00116601
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DOI: https://doi.org/10.1007/BF00116601