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Microbial degradation of pentachlorophenol

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Abstract

Pentachlorophenol (PCP) was the most prevalent wood preservative for many years worldwide. Its widespread use had led to contamination of various environments. Traditional methods of PCP clean-up include storage in land-fill sites, incineration and abiotic degradation processes such as photodecomposition. Some aerobic and anaerobic microorganisms can degrade PCP under a variety of conditions. Axenic bacterial cultures, Flavobacterium sp., Rhodococcus sp., Arthrobacter sp., Pseudomonas sp., Sphingomonas sp., and Mycobacterium sp., and fungal cultures, Phanerochaete sp. and Trametes sp. exhibit varying rates and extent of PCP degradation. This paper provides some general information on properties of PCP and reviews the influence of nutrient amendment, temperature and pH on PCP degradation by various aerobic and anaerobic microorganisms. Where information is available, proposed degradation pathways, intermediates and enzymes are reviewed.

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References

  • Abrahamsson, K & Klick, S (1991) Degradation of halogenated phenols in anoxic natural marine sediments. Mar. Pollut. Bull. 22: 227–233

    Google Scholar 

  • Alleman, BC, Logan, BE & Gilbertson, RL (1992) Toxicity of pentachlorophenol to six species of white rot fungi as a function of chemical dose. Appl. Environ. Microbiol. 58: 4048–4050

    Google Scholar 

  • Alleman, BC, Logan, BE & Gilbertson, RL (1993) A rapid method to screen fungi for resistance to toxic chemicals. Biodegradation 4: 125–129

    Google Scholar 

  • Apajalahti, JHA, Krpnoja, P & Salkinoja-Salonen, MS (1986) Rhodococcus chlorophenolicus sp. nov., a chlorophenolmineralizing actinomycete. Int. J. System. Bacteriol. 36: 246–251

    Google Scholar 

  • Apajalahti, JHA & Salkinoja-Salonen, MS (1984) Absorption of pentachlorophenol (PCP) by bark chips and its role in microbial PCP degradation. Microb. Ecol. 10: 359–367

    Google Scholar 

  • Apajalahti, JHA & Salkinoja-Salonen, MS (1986) Degradation of polychlorinated phenols by Rhodococcus chlorophenolicus. Appl. Microbiol. Biotechnol. 25: 62–67

    Google Scholar 

  • Apajalahti, JHA & Salkinoja-Salonen, MS (1987) Dechlorination and para-hydroxylation of polychlorinated phenols by Rhodococcus chlorophenolicus. J. Bacteriol. 169: 675–681

    Google Scholar 

  • Bellin, CA & O'Connor, GA (1990) Plant uptake of pentachlorophenol from sludge-amended soils. J. Environ. Qual. 19: 598–602

    Google Scholar 

  • Bellin, CA, O'Connor, GA & Jin, Y (1990) Sorption and degradation of pentachlorophenol in sludge-amended soils. J. Environ. Qual. 19: 603–608

    Google Scholar 

  • Briglia, M, Eggen, RI, VanElsas, JD & deVos, WM (1994) Phylogenetic evidence for transfer of pentachlorophenol-mineralizing Rhodococcus chlorophenolicus strain PCP-1 to the genus Mycobacterium. Int. J. Syst. Bacteriol. 44: 494–498

    Google Scholar 

  • Briglia, M, Middeldorp, PJM & Salkinoja-Salonen, MS (1994) Mineralization performance of Rhodococcus chlorophenolicus strain PCP-1 in contaminated soil simulating on site conditions. Soil. Biol. Biochem. 26: 377–385

    Google Scholar 

  • Briglia, M, Nurmiaho-Lassila, E-L, Vallini, G & Salkinoja-Salonen, MS (1990) The survival of the pentachlorophenol-degrading Rhodococcus chlorophenolicus PCP-1 and Flavobacterium sp. in natural soil. Biodegradation 1: 273–281

    Google Scholar 

  • Brown, EJ, Pignatello, JJ, Martinson, MM & Crawford, RL (1986) Pentachlorophenol degradation: a pure bacterial culture and epilithic microbial consortium. Appl. Environ. Microbiol. 52: 92–97

    Google Scholar 

  • Bryant, FO, Hale, DD & Rogers, JE (1991) Regiospecific dechlorination of pentachlorophenol by dichlorophenol-adapted microorganisms in freshwater, anaerobic sediment slurries. Appl. Environ. Microbiol. 57: 2293–2301

    Google Scholar 

  • Bryant, SE & Schultz, TW (1994) Toxicological assessment of biotransformation products of pentachlorophenol: Tetrahymena population growth impairment. Arch. Environ. Contam. Toxicol. 26: 299–303

    Google Scholar 

  • Cassidy MB, Leung K, Lee H & Trevors JT (1995) Survival of laclux-marked Pseudomonas aeruginosa UG2Lr cells encapsulated in k-carrageenan and alginate. J. Microbiol. Meth. (in press)

  • Casterline, JL, Barnett, NM & Ku, Y (1985) Uptake, translocation, and transformation of pentachlorophenol in soybean and spinach plants. Environ. Res. 37: 101–118

    Google Scholar 

  • Chu, JP & Kirsch, EJ (1972) Metabolism of pentachlorophenol by axenic bacterial culture. Appl. Environ. Microbiol. 23: 1033–1035

    Google Scholar 

  • Crawford, RL & Mohn, WW (1985) Microbiological removal of pentachlorophenol from soil using a Flavobacterium. Enzyme Microb. Technol. 7: 617–620

    Google Scholar 

  • Crosby, DG (1981) Environmental chemistry of pentachlorophenol. Pure Appl. Chem. 53: 1051–1080

    Google Scholar 

  • Deweerd, KA, Mandelco, L, Tanner, RS, Woese, CR & Suflita, JM (1990) Desulfomonile tiedjei gen. nov. and sp. nov., a novelanaerobic, dehalogenating, sulfate-reducing bacterium. Arch. Microbiol. 154: 23–30

    Google Scholar 

  • Dietrich, G & Winter, J (1990) Anaerobic degradation of chlorophenol by an enrichment culture. Appl. Microbiol. Biotechnol. 34: 253–258

    Google Scholar 

  • Edgehill, RU (1994) Pentachlorophenol removal from slightly acidic mineral salts, commercial sand, and clay soil by recovered Arthrobacter strain ATCC 33790. Appl. Microbiol. Biotechnol. 41: 142–148

    Google Scholar 

  • Edgehill, RU & Finn, RK (1983) Microbial treatment of soil to remove pentachlorophenol. Appl. Environ. Microbiol. 45: 1122–1125

    Google Scholar 

  • Erickson, BD & Mondello, FJ (1993) Enhanced biodegradation of polychlorinated biphenyls after site-directed mutagenesis of a biphenyl dioxygenase gene. Appl. Environ. Microbiol. 59: 3858–3862

    Google Scholar 

  • Frank, R, Braun, HE, Stonefield, KI, Rasper, J & Luyken, H (1990) Organochlorine and organophosphorus residues in the fat of domestic farm animals species, Ontario, Canada 1986–1988. Food Addit. Contam. 7: 629–636

    Google Scholar 

  • Gilbert, FI, Minn, CE, Duncan, RC & Wilkinson, J (1990) Effects of pentachlorophenol and other chemical preservatives on the health of wood-treating workers in Hawaii. Arch. Environ. Contam. Toxicol. 19: 603–609

    Google Scholar 

  • Gonzalez, JF & Hu, W-S (1991) Effect of glutamate on the degradation of pentachlorophenol by Flavobacterium sp. Appl. Microbiol. Biotechnol. 35: 100–104

    Google Scholar 

  • Guthrie, MA, Kirsch, EJ, Wukasch, RF & Grady, CPL (1984) Pentachlorophenol biodegradation II-Anaerobic. Water Res. 18: 451–461

    Google Scholar 

  • Häggblom, MM, Apajalahti, JHA & Salkinoja-Salonen, MS (1988a) Hydroxylation and dechlorination of chlorinated guaiacols and syringols by Rhodococcus chlorophenolicus. Appl. Environ. Microbiol. 54: 683–688

    Google Scholar 

  • Häggblom, MM, Apajalahti, JHA & Salkinoja-Salonen, MS (1988b) O-methylation of chlorinated para-hydroquinones by Rhodococcus chlorophenolicus. Appl. Environ. Microbiol. 54: 1818–1824

    Google Scholar 

  • Häggblom, MM, Nohynek, LJ & Salkinoja-Salonen, MS (1988c) Degradation and O-methylation of chlorinated phenolic compounds by Rhocococcus and Mycobacterium strains. Appl. Environ. Microbiol. 54: 3043–3052

    Google Scholar 

  • Häggblom, MM, Janke, D & Salinoja-Salonen, MS (1989) Hydroxylation and dechlorination of tetrachlorohydroquinone by Rhodococcus sp. strain CP-2 cell extracts. Appl. Environ. Microbiol. 55: 516–519

    Google Scholar 

  • Häggblom, MM & Young, LY (1990) Chlorophenol degradation coupled to sulfate reduction. Appl. Environ. Microbiol. 56: 3255–3260

    Google Scholar 

  • Hendriksen, HV & Ahring, BK (1993) Anaerobic dechlorination of pentachlorophenol in fixed-film and upflow anaerobic sludge blanket reactors using different inocula. Biodegradation 3: 399–408

    Google Scholar 

  • Hendriksen, HV, Larsen, S & Ahring, BK (1992) Influence of a supplemental carbon source on anaerobic dechlorination of pentachlorophenol in granular sludge. Appl. Environ. Microbiol. 58: 365–370

    Google Scholar 

  • Higson, FK (1991) Degradation of xenobiotics by white-rot fungi. In: Ware, GW & FAGunther (Eds) Reviews of Environmental Contamination and Toxicology (pp. 111–152) Springer-Verlag, New York

    Google Scholar 

  • Hu, ZC, Korus, RA, Levinson, WE & Crawford, RL (1994) Adsorption and biodegradation of pentachlorophenol by polyurethaneimmobilized Flavobacterium. Environ. Sci. Technol. 28: 491–496

    Google Scholar 

  • Izaki, K, Takahashi, M, Sato, Y, Sasagawa, Y, Sato, K & Furusaka, C (1981) Some properties of pentachlorophenol-resistant gramnegative bacteria. Agric. Biol. Chem. 45: 765–767

    Google Scholar 

  • Jacobsen, BN, Nyholm, N, Pedersen, BM, Poulsen, O & [SS];stfeldt, P (1991) Microbial degradation of pentachlorophenol and lindane in laboratory-scale activated sludge reactors. Water Sci. Technol. 23: 349–356

    Google Scholar 

  • Jacobsen BN, Nyholm N, Pedersen BM, Poulsen O & [SS];stfeldt P (1993) Removal of organic micropollutants in laboratory activated sludge reactors under various operating conditions: sorption. Water Res. 1505–1510

  • Järvinen, KT, Melin, ES & Puhakka, JA (1994) High-rate bioremediation of chlorophenol-contaminated groundwater at low temperatures. Environ. Sci. Technol. 28: 2387–2392

    Google Scholar 

  • Järvinen, KT & Puhakka, JA (1994) Bioremediation of chlorophenol contaminated ground water. Environ. Technol. 15: 823–832

    Google Scholar 

  • Jekat, FW, Meisel, ML, Eckard, R & Winterhoff, H (1994) Effects of pentachlorophenol (PCP) on the pituitary and thyroidal hormone regulation in the rat. Toxicol. Lett. 71: 9–25

    Google Scholar 

  • Johri, S, Qazi, GN & Chopra, CL (1991) Evidence of plasmid mediated dechlorinase activity in Pseudomonas sp. J. Biotechnol. 20: 73–82

    Google Scholar 

  • Kitunen, VH, Valo, RJ & Salkinoja-Salonen, MS (1987) Contamination of soil around wood-preserving facilities by polychlorinated aromatic compounds. Environ. Sci. Technol. 21: 101

    Google Scholar 

  • Klecka, GM & Maier, WJ (1985) Kinetics of microbial growth on pentachlorophenol. Appl. Environ. Microbiol. 49: 46–53

    Google Scholar 

  • Kohring, G-W, Zhang, X & Wiegel, J (1989) Anaerobic dechlorination of 2,4-dichlorophenol in fresh water sediments in the presence of sulfate. Appl. Environ. Microbiol. 55: 2735–2737

    Google Scholar 

  • Krumme, ML & Boyd, SA (1988) Reductive dechlorination of chlorinated phenols in anaerobic upflow bioreactors. Water Res. 22: 171–177

    Google Scholar 

  • Kuwatsuka, S & Igarashi, M (1975) Degradation of PCP in soils. Soil Sci. Plant Nutr. 21: 405–414

    Google Scholar 

  • Lamar, RT & Dietrich, DM (1990) In situ depletion of pentachlorophenol from contaminated soil by Phanerochaete spp. Appl. Environ. Microbiol. 56: 3093–3100

    Google Scholar 

  • Lamar, RT & Dietrich, DM (1992) Use of lignin-degrading fungi in the disposal of pentachlorophenol-treated wood. J. Ind. Microbiol. 9: 181–191

    Google Scholar 

  • Lamar, RT & Evans, JW (1993) Solid-phase treatment of a pentachlorophenol-contaminated soil using lignin-degrading fungi. Environ. Sci. Technol. 27: 2566–2571

    Google Scholar 

  • Lamar, RT, Glaser, JA & Kirk, TK (1990a) Fate of pentachlorophenol (PCP) in sterile soils inoculated with the white-rot Basidiomycete Phanerochaete chrysosporium: mineralization, volatilization, and depletion of PCP. Soil Biol. Biochem. 22: 433–440

    Google Scholar 

  • Lamar, RT, Larsen, MJ & Kirk, TK (1990b) Sensitivity to and degradation of pentachlorophenol by Phanerochaete spp. Appl. Environ. Microbiol. 56: 3519–3526

    Google Scholar 

  • Larsen, S, Hendriksen, HV & Ahring, BK (1991) Potential for thermophilic (50C) anaerobic dechlorination of pentachlorophenol in different ecosystems. Appl. Environ. Microbiol. 57: 2085–2090

    Google Scholar 

  • Larsson, P & Lemkemeier, K (1989) Microbial mineralization of chlorinated phenols and biphenyls in sediment-water systems from humic and clear-water lakes. Water Res. 23: 1081–1085

    Google Scholar 

  • Lin, JE, Wang, HY & Hickey, RF (1990) Degradation kinetics of pentachlorophenol by Phanerochaete chrysosporium. Biotechnol. Bioeng. 35: 1125–1134

    Google Scholar 

  • Lin, JE, Wang, HY & Hickey, RF (1991) Use of co-immobilized biological systems to degrade toxic organic compounds. Biotechnol. Bioeng. 38: 273–279

    Google Scholar 

  • Liu, D (1989) Biodegradation of pentachlorophenol and its commercial formulation. Tox. Assess. 4: 115–127

    Google Scholar 

  • Liu, D, Maguire, RJ, Pacepavicius, G & Dutka, BJ (1991) Biodegradation of recalcitrant chlorophenols by cometabolism. Environ. Toxicol. Water Qual. 6: 85–95

    Google Scholar 

  • Madsen, T & Aamand, J (1991) Effects of sulfuroxy anions on degradation of pentachlorophenol by a methanogenic enrichment culture. Appl. Environ. Microbiol. 57: 2453–2458

    Google Scholar 

  • McBain, A, Cui, F, Herbert, L & Ruddick, JNR (1995) The microbial degradation of chlorophenolic preservatives in spent, pressuretreated timber. Biodegradation 6: 47–55

    Google Scholar 

  • Michel, FC, Dass, SB, Grulke, EA & Reddy, CA (1991) Role of manganese peroxidases and lignin peroxidases of Phanerochaete chrysosporium in the decolorization of kraft bleach plant effluent. Appl. Environ. Microbiol. 57: 2368–2375

    Google Scholar 

  • Middeldorp, PJM, Briglia, M & Salkinoja-Salonen, MS (1990) Biodegradation of pentachlorophenol in natural soil by inoculated Rhodococcus chlorophenolicus. Microb. Ecol. 20: 123–139

    Google Scholar 

  • Mikesell, MD & Boyd, SA (1986) Complete reductive dechlorination and mineralization of pentachlorophenol by anaerobic microorganisms. Appl. Environ. Microbiol. 52: 861–865

    Google Scholar 

  • Mikesell, MD & Boyd, SA (1988) Enhancement of pentachlorophenol degradation in soil through induced anaerobiosis and bioaugmentation with anaerobic sewage sludge. Environ. Sci. Technol. 22: 1411–1414

    Google Scholar 

  • Mileski, GJ, Bumpus, JA, Jurek, MA & Aust, SD (1988) Biodegrdadation of pentachlorophenol by the white rot fungus Phanerochaete chrysosporium. Appl. Environ. Microbiol. 54: 2885–2889

    Google Scholar 

  • Mills, G & Hoffmann, MR (1993) Photocatalytic degradation of pentachlorophenol on TiO2 particles: identification of intermediates and mechanism of reaction. Environ. Sci. Technol. 27: 1681–1689

    Google Scholar 

  • Mohn, WW & Kennedy, KJ (1992a) Reductive dehalogenation of chlorophenols by Desulfomonile tiedjei DCB-1. Appl. Environ. Microbiol. 58: 1367–1370

    Google Scholar 

  • Mohn, WW & Kennedy, KJ (1992b) Limited degradation of chlorophenols by anaerobic sludge granules. Appl. Environ. Microbiol. 58: 2131–2136

    Google Scholar 

  • Moos, LP, Kirsch, EJ, Wukasch, RF & Grady, CPL (1983) Pentachlorophenol biodegradation-1 Aerobic. Water Res. 17: 1575–1584

    Google Scholar 

  • Mueller, JG, Lantz, SE, Ross, D, Colvin, RJ, Middaugh, DP & Pritchard, PH (1993) Strategy using bioreactors and specially selected microorganisms for bioremediation of groundwater contaminated with creosote and pentachlorophenol. Environ. Sci. Technol. 27: 691–698

    Google Scholar 

  • Mueller, JG, Middaugh, DP, Lantz, SE & Chapman, PJ (1991) Biodegradation of Creosote and pentachlorophenol in contaminated groundwater: chemical and biological assessment. Appl. Environ. Microbiol. 57: 1277–1285

    Google Scholar 

  • Nevalainen, I, Kostyl, E, Nurmiaho-Lassila, E-L, Puhakka, JA & Salkinoja-Salonen, MS (1993) Dechlorination of 2,4,6-trichlorophenol by a nitrifying biofilm. Water Res. 27: 757–767

    Google Scholar 

  • Nicholson, DK, Woods, SL, Istok, JD & Peek, DC (1992) Reductive dechlorination of chlorophenols by a pentachlorophenol-acclimated methanogenic consortium. Appl. Environ. Microbiol. 58: 2280–2286

    Google Scholar 

  • Nwoga, J & Bittar, E (1991) An investigation of the sensitivity of the ouabain-insensitive sodium efflux in single barnacle muscle fibers to pentachlorophenol. Toxicol. Appl. Pharmacol. 108: 330–341

    Google Scholar 

  • O'Reilly, KT & Crawford, RL (1989) Degradation of pentachlorophenol by polyurethane-immobilized Flavobacterium cells. Appl. Environ. Microbiol. 55: 2113–2118

    Google Scholar 

  • Orser, CS, Lange, CC, Xun, L, Zahrt, TC & Schneider, BJ (1993a) Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli. J. Bacteriol. 175: 411–416

    Google Scholar 

  • Orser, CS, Dutton, J, Lange, C, Jablonski, P, Xun, L & Hargis, M (1993b) Characterization of a Flavobacterium glutathione Stransferase gene involved in reductive dechlorination. J. Bacteriol. 175: 2640–2644

    Google Scholar 

  • Périé, FH & Gold, MH (1991) Manganese regulation of manganese peroxidase expression and lignin degradation by the white rot fungus Dichomitus squalens. Appl. Environ. Microbiol. 57: 2240–2245

    Google Scholar 

  • Pignatello, JJ, Martinson, MM, Steiert, JG, Carlson, RE & Crawford, RL (1993) Biodegradation and photolysis of pentachlorophenol in artificial freshwater streams. Appl. Environ. Microbiol. 46: 1024–1031

    Google Scholar 

  • Puhakka, JA & Järvinen, K (1992) Aerobic fluidized-bed treatment of polychlorinated phenolic wood preservative constituents. Water Res. 26: 765–770

    Google Scholar 

  • Radehaus, PM & Schmidt, SK (1992) Characterization of a novel Pseudomonas sp. that mineralizes high concentrations of pentachlorophenol. Appl. Environ. Microbiol. 58: 2879–2885

    Google Scholar 

  • Rao, KR (1978) Pentachlorophenol: Chemistry, Pharmacology, and Environmental Toxicology. (pp. 3–18) Plenum Press, New York

    Google Scholar 

  • Roy-Arcand, L & Archibald, FS (1991) Direct dechlorination of chlorophenolic compounds by laccases from Trametes (Coriolus) versicolor. Enzyme Microb. Technol. 13: 194–203

    Google Scholar 

  • Ruckdeschel, G, Renner, G & Schwartz, K (1987) Effects of pentachlorophenol and some of its known and possible metabolites on different species of bacteria. Appl. Environ. Microbiol. 53: 2689–2692

    Google Scholar 

  • Ruddick, JNR (1991) Utility pole performance: pentachlorophenol distribution and content in recovered pine poles. Wood Protect. 1: 77–83

    Google Scholar 

  • Rutgers, M, Bogte, JJ, Breure, AM & vanAndel, JG (1993) Growth and enrichment of pentachlorophenol-degrading microorganisms in the nutristat, a substrate concentration-controlled continuous culture. Appl. Environ. Microbiol. 59: 3373–3377

    Google Scholar 

  • Saber, DL & Crawford, RL (1985) Isolation and characterization of Flavobacterium strains that degrade pentachlorophenol. Appl. Environ. Microbiol. 50: 1512–1518

    Google Scholar 

  • Salkinoja-Salonen, MS (1990) Biochemistry and ecology of the clean-up of pentachlorophenol from contaminated soils. Fifth Colloquium on Pulp and Paper Mill Effluents Sato K (1983) Effect of a pesticide, pentachlorophenol (PCP) on soil microflora. Plant & Soil 75: 417–426

    Google Scholar 

  • Salkinoja-Salonen, MS (1985) Effect of a pesticide, pentachlorophenol (PCP) on soil microflora. II Effect of PCP on bacterial flora in soil percolated with glycine or water. J. Gen. Appl. Microbiol. 31: 197–210

    Google Scholar 

  • Salkinoja-Salonen, MS (1987) Effect of increasing pentachlorophenol (PCP) concentrations on bacterial populations in glycine-percolated soils. Biol. Fertil. Soils 5: 1–5

    Google Scholar 

  • Schenk, T, Mller, R & Lingens, F (1990) Mechanisms of enzymatic dehalogenation of pentachlorophenol by Arthrobacter sp. strain ATCC 33790. J. Bacteriol. 172: 7272–7274

    Google Scholar 

  • Schenk, T, Mller, R, Mrsberger, F, Otto, MK & Lingens, F (1989) Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790. J. Bacteriol. 177: 5487–5491

    Google Scholar 

  • Scheunert, I, Qiao, Z & Korte, F (1986) Comparative studies of the fate of atrazine-14C and pentachlorophenol-14C in various laboratory and outdoor soil-plant systems. J. Environ. Sci. Health B21: 457–485

    Google Scholar 

  • Seech, AG, Marvan, IJ & Trevors, JT (1994) On site/ex situ bioremediation of industrial soils containing chlorinated phenols and polycyclic aromatic hydrocarbons. In: Hinchee, RE, Leeson, A, Semprini, L & Ong, SK (Eds) Bioremediation of Chlorinated and Polycyclic Aromatic Hydrocarbon Compounds (pp. 451–455) Lewis Publishers, Boca Raton, Florida

    Google Scholar 

  • Seech, AG, Trevors, JT & Bulman, TL (1991) Biodegradation of pentachlorophenol in soil: the response of physical, chemical, and biological treatments. Can. J. Microbiol. 37: 440–444

    Google Scholar 

  • Seigle-Murandi, F, Steiman, R & Benoit-Guyod, JL (1991) Biodegradation potential of some micromycetes for pentachlorophenol. Ecotox. Environ. Safety 21: 290–300

    Google Scholar 

  • Seigle-Murandi, F, Steiman, R, Benoit-Guyod, JL & Guiraud, P (1993) Fungal degradation of pentachlorophenol by micromycetes. J. Biotechnol. 30: 27–35

    Google Scholar 

  • Shimizu, Y, Yamazaki, S & Terashima, Y (1992) Sorption of anionic pentachlorophenol (PCP) in aquatic environments: the effect of pH. Water Sci. Technol. 25: 41–48

    Google Scholar 

  • Siahpush, AR, Lin, JE & Wang, HY (1992) Effect of adsorbents on degradation of toxic organic compounds by coimmobilized systems. Biotechnol. Bioeng. 39: 619–628

    Google Scholar 

  • Slater, JH, Lovatt, D, Weightman, AJ, Senior, E & Bull, AT (1979) The growth of Pseudomonas putida on chlorinated aliphatic acids and its dehalogenase activity. J. Gen. Microbiol. 114: 125–136

    Google Scholar 

  • Smejtek, P, Barstad, AW & Wang, S (1989) Pentachlorophenolinduced change of θ-potential and gel-to-fluid transition temperature in model lecithin membranes. Chem. Biolog. Interact. (1989) 71: 37–61

    Google Scholar 

  • Stanlake, GJ & Finn, RK (1982) Isolation and characterization of a pentachlorophenol-degrading bacterium. Appl. Environ. Microbiol. 44: 1421–1427

    Google Scholar 

  • Steiert, JG & Crawford, RL (1986) Catabolism of pentachlorophenol by a Flavobacterium sp. Biochem. Biophys. Res. Commun. 141: 825–830

    Google Scholar 

  • Steiert, JG, Pignatello, JJ & Crawford, RL (1987) Degradation of chlorinated phenols by a pentachlorophenol-degrading bacterium. Appl. Environ. Microbiol. 53: 907–910

    Google Scholar 

  • Steiert, JG, Thoma, WJ, Ugurbil, K & Crawford, RL (1988) 31P Nuclear magnetic resonance studies of effects of some chlorophenols on Escherichia coli and a pentachlorophenol-degrading bacterium. J. Bacteriol. 170: 4954–4957

    Google Scholar 

  • Suzuki, T (1978) Enzymatic methylation of pentachlorophenol and its related compounds by cell-free extracts of Mycobacterium sp. isolated from soil. J. Pesticide Sci. 3: 441–443

    Google Scholar 

  • Suzuki, T (1983) Methylation and hydroxylation of pentachlorophenol by Mycobacterium sp. isolated from soil. J. Pesticide Sci. 8: 419–428

    Google Scholar 

  • Topp, E, Crawford, RL & Hanson, RS (1988) Influence of a readily metabolizable carbon on pentachlorophenol metabolism by a pentachlorophenol-degrading Flavobacterium sp. Appl. Environ. Microbiol. 54: 2452–2459

    Google Scholar 

  • Topp, E & Hanson, RS (1990a) Factors influencing the survival and activity of a pentachlorophenol-degrading Flavobacterium sp. in soil slurries. Can. J. Soil Sci. 70: 83–91

    Google Scholar 

  • Topp, E & Hanson, RS (1990b) Degradation of pentachlorophenol by a Flavobacterium species grown in continuous culture under various nutrient limitations. Appl. Environ. Microbiol. 56: 541–544

    Google Scholar 

  • Topp, E, Xun, L & Orser, CS (1992) Biodegradation of the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) by purified pentachlorophenol hydroxylase and whole cells of Flavobacterium sp. strain ATC 39723 is accompanied by cyanogenesis. Appl. Environ. Microbiol. 58: 502–506

    Google Scholar 

  • Trevors, JT (1982) Effect of temperature on the degradation of pentachlorophenol by Pseudomonas species. Chemosphere 11: 471–475

    Google Scholar 

  • Trevors, JT (1983) Effect of pentachlorophenol on the membrane fluidity of Pseudomonas fluorescens. FEMS Microbiol. Lett. 16: 331–334

    Google Scholar 

  • Trevors, JT, Mayfield, CI & Innis, WE (1982) Effect of the sequence of exposure to chlorophenols in short-term bacterial bioassays. Arch. Environ. Contam. Toxicol. 11: 203–207

    Google Scholar 

  • Trevors, JT, VanElsas, JD, Lee, H & VanOverbeek, LS (1992) Use of alginate and other carriers for encapsulation of microbial cells for use in soil. Microb. Release 1: 61–69

    Google Scholar 

  • Uotila, JS, Kitunen, VH, Apajahlahti, JHA & Salkinoja-Salonen, MS (1992) Environment-dependent mechanism of dehalogenation by Rhodococcus chlorophenolicus PCP-1. Appl. Microbiol. Biotechnol. 38: 408–412

    Google Scholar 

  • Uotila, JS, Salkinoja-Salonen, MS & Apajalahti, JHA (1991) Dechlorination of pentachlorophenol by membrane bound enzymes of Rhodococcus chlorophenolicus PCP-1. Biodegradation 2: 25–31

    Google Scholar 

  • Valo, RJ, Apajalahti, J & Salkinoja-Salonen, MS (1985) Studies on the physiology of microbial degradation of pentachlorophenol. Appl. Microbiol. Biotechnol. 21: 313–319

    Google Scholar 

  • Valo, R, Kitumen, V, Salkinoja-Salonen, MS & Risnen, S (1984) Chlorinated phenols as contaminants of soil and water in the vicinity of two Finnish sawmills. Chemosphere 13: 835–844

    Google Scholar 

  • Wall, AJ & Stratton, GW (1994) Effects of a chromated-copper arsenate wood preservative on the bacterial degradation of pentachlorophenol. Can. J. Microbiol. 40: 388–392

    Google Scholar 

  • Watanabe, I (1977) Pentachlorophenol-decomposing and PCP-tolerant bacteria in field soil treated with PCP. Soil Biol. Biochem. 9: 99–103

    Google Scholar 

  • Watanabe, I (1978) Pentachlorophenol (PCP) decomposing activity of field soils treated annually with PCP. Soil Biol. Biochem. 10: 71–75

    Google Scholar 

  • Wegman, RCC & van denBroek, HH (1983) Chlorophenols in river sediment in the Netherlands. Water Res. 17: 227–230

    Google Scholar 

  • Wegman, RCC & Hofstee, AWM (1979) Chlorophenols in surface waters of the Netherlands (1976–1977). Water Res. 13: 651–657

    Google Scholar 

  • Wester, RC, Maibach, HI, Sedik, L, Melendres, J, Wade, M & Dizio, S (1993) Percutaneous absorption of pentachlorophenol from soil. Fundam. Appl. Toxicol. 20: 68–71

    Google Scholar 

  • Wild, SR, Harrad, SJ & Jones, KC (1993) Chlorophenols in digested U.K. sewage sludges. Water Res. 27: 1527–1534

    Google Scholar 

  • Woods, SL, Ferguson, JF & Benjamin, MM (1989) Characterization of chlorophenol and chloromethoxybenzene biodegradation during anaerobic treatment. Environ. Sci. Technol. 23: 62–68

    Google Scholar 

  • Wu, W-M, Bhatnagar, L & Zeikus, JG (1993) Performance of anaerobic granules for degradation of pentachlorophenol. Appl. Environ. Microbiol. 59: 389–397

    Google Scholar 

  • Xun, L & Orser, CS (1991a) Biodegradation of triiodophenol by cell-free extracts of a pentachlorophenol-degrading Flavobacterium sp. Biochem. Biophys. Res. Commun. 174: 43–48

    Google Scholar 

  • Xun, L & Orser, CS (1991b) Purification of a Flavobacterium pentachlorophenol-induced periplasmic protein (PcpA) and nucleotide sequence of the corresponding gene (pcpA). J. Bacteriol. 173: 2920–2926

    Google Scholar 

  • Xun, L & Orser, CS (1991c) Purification and properties of pentachlorophenol hydroxylase, a flavoprotein from Flavobacterium sp. strain ATCC 39723. J. Bacteriol. 173: 4447–4453

    Google Scholar 

  • Xun, L, Topp, E & Orser, CS (1992a) Glutathione is the reducing agent for the reductive dehalogenation of tetrachloro-p-hydroquinone by extracts from a Flavobacterium sp. Biochem. Biophys. Res. Commun. 182: 361–366

    Google Scholar 

  • Xun, L, Topp, E & Orser, CS (1992b) Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries. J. Bacteriol. 174: 2898–2902

    Google Scholar 

  • Xun, L, Topp, E & Orser, CS (1992c) Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase. J. Bacteriol. 174: 5745–5747

    Google Scholar 

  • Xun, L, Topp, E & Orser, CS (1992d) Purification and characterization of a tetrachloro-p-hydroquinone reductive dehalogenase from a Flavobacterium sp. J. Bacteriol. 174: 8003–8007

    Google Scholar 

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  1. Department of Microbiology, University of Guelph, NIG 2W1, Guelph, Ontario, Canada

    Kelly A. McAllister

  2. Department of Environmental Biology, University of Guelph, NIG 2W1, Guelph, Ontario, Canada

    Hung Lee & Jack T. Trevors

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  1. Kelly A. McAllister
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  2. Hung Lee
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McAllister, K.A., Lee, H. & Trevors, J.T. Microbial degradation of pentachlorophenol. Biodegradation 7, 1–40 (1996). https://doi.org/10.1007/BF00056556

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