Abstract
In an attempt to solve the controversy about the evaluation of the molar absorption coefficient of PChl(ide), this coefficient is estimated in this work by using an original experimental approach. The calculated molar absorption coefficient of PChl(ide) is 30.4.103 1 mole−1 cm−1 at 626 nm in acetone 80%; it is close to that derived from the specific absorption coefficient of Koski and Smith when assuming that the pigment extracted by these authors was the esterified pigment: PChl. Sets of equations for the quantification of Chl(ide) a, Chl b and PChl(ide) in 80% acetone extracts are derived.
Similar content being viewed by others
Abbreviations
- PChl(ide):
-
protochlorophyll(ide)
- Chl(ide):
-
chlorophyll(ide)
References
Anderson JM and Boardman NK (1964) Studies on the greening of dark-grown bean plants. Aust J Biol Sci 17:93–101
Boardman NK (1966) Protochlorophyll. In Vernon LP and Seely GR; eds. The chlorophylls, pp. 437–479. Academic Press New York and London
Granick S (1950) Magnesium vinyl pheophorphyrin a5. Another intermediate in the biological synthesis of chlorophyll. J Biol Chem 183:713–730
Holt AS and Jacobs EE (1954) Spectroscopy of plant pigments I — Ethylchlorophyllides a and b and their pheophorbides. Amer J Bot 41:710–717
Houssier C and Sauer K (1969) Optical properties of the protochlorophyll pigments II: Electronic absorption, fluorescence and circular dichroism spectra. Biochem Biophys acta 172:492–502
Kahn A (1983) Spectrophotometric quantitation of protochlorophyll(ide): extinction coefficients reconsidered. Physiol Plant (submitted for publication)
Koski VM and Smith JHC (1948). The isolation and spectral absorption propertoes of protochlorophyll from etiolated barley seedlings. J Am Chem Soc 70:3558–3562
Loeffler JE (1955) Precursors of protochlorophyll in etiolated barley seedlings. Carnegie Inst Wash Year Book, 54:159–160
MacKinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322
Šesták Z (1971) Determination of chlorophylls a and b. In Šesták Z et al: eds. Plant photosynthetic production. Manual of methods, pp. 672–701. The Hague: Junk
Sironval C, Michel-Wolwertz M-R and Madsen A (1965) On the nature and possible functions of the 673 and 684 nm forms in vivo of chlorophyll. Biochem Biophys Acta 94:344–354
Svec WA (1978) The isolation, preparation, characterization and estimation of the chlorophylls and the bacteriochlorophylls. In Dolphin D eds. The Porphyrins vol. V, pp. 341–399. Academic Press New York, San Francisco London
Vernon LP (1960) Spectrophotometric determination of chlorophylls and pheophytins in plant extracts. Anal Chem 32:1144–1150
Wintermans JFGM and de Mots A (1965) Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol Biochim Biophys Acta 109: 448–453
Wolff JB and Price L (1957) Terminal steps of chlorophyll a biosynthesis in higher plants. Arch Biochem Biophys 72:293–301
Ziegler R and Egle K (1965) Zur quantitativen analyse der chloroplastenpigmente I Kritische Überprüfung der spektralphotometrischen chlorophyll-Bestimmung Beitr Biol Pflanzen 41:11–37
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brouers, M., Michel-Wolwertz, MR. Estimation of protochlorophyll(ide) contents in plant extracts; re-evaluation of the molar absorption coefficient of protochlorophyll(ide). Photosynth Res 4, 265–270 (1983). https://doi.org/10.1007/BF00052130
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00052130