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Extraction and Purification of Phlorotannins from Brown Algae

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Natural Products From Marine Algae

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1308))

Abstract

The interest in the physiological roles and bioactivities of plant phenols has increased over the past decades. In seaweeds, many investigations have dealt with phenolic compounds of Phaeophyceae (phlorotannins), even though little is known so far about the ecophysiological variations of their pool or their biosynthetic pathways. We describe here a simple procedure based on the use of water-organic solvent mixtures for the extraction of phlorotannins. Crude extracts are semi-purified and fractionated by separating methods based on both the polarity and the molecular size of compounds. Phenols are then quantified by the Folin-Ciocalteu method and their radical-scavenging activity is characterized using the DPPH test. All along the purification process of phenolic compounds, the efficiency of separation is assessed by 1H-NMR.

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Acknowledgements

This work was supported by a grant-in-aid for scientific research (Phlorotan’ING project) from the “Groupement d’Intérêt Scientifique” Europôle Mer to V.S.P. and fellowships from the French Ministry for National Education, Higher Education and Research to M.H. and C.J. The authors are grateful to S. Cérantola, N. Kervarec, and G. Simon for support in NMR analysis and to E. Charpentier.

Author information

Authors and Affiliations

  1. LEMAR–UMR6539, IUEM, str. Dumont D’Urville, Plouzané, Brittany, 29280, France

    Erwan Ar Gall & Valérie Stiger-Pouvreau

  2. University of Brest (University of Western Brittany – UEB), Brest, France

    Erwan Ar Gall, Camille Jégou & Valérie Stiger-Pouvreau

  3. BMM Laboratory, Ifremer-Brest, ZI Began Diaoul, Plouzané, Brittany, 29280, France

    Florian Lelchat

  4. Salipouss, 76 bis route du port, Fouesnant, Brittany, 29170, France

    Mélanie Hupel

  5. Lubem Quimper–EA3882, 6 rue de l’Université, Quimper, Brittany, 29000, France

    Camille Jégou

Authors
  1. Erwan Ar Gall
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  2. Florian Lelchat
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  3. Mélanie Hupel
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  4. Camille Jégou
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  5. Valérie Stiger-Pouvreau
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Corresponding author

Correspondence to Erwan Ar Gall .

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Editors and Affiliations

  1. Botany and Plant Science, School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research,, National University of Ireland Galway, Galway, Ireland

    Dagmar B. Stengel

  2. Photobiotechnology, INTECHMER, Conservatoire National des Arts et Métiers, Cherbourg, Cedex, France, CNRS, GEPEA, UMR6144, Boulevard de l’Université, Saint Nazaire, Cedex, France

    Solène Connan

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Gall, E.A., Lelchat, F., Hupel, M., Jégou, C., Stiger-Pouvreau, V. (2015). Extraction and Purification of Phlorotannins from Brown Algae. In: Stengel, D., Connan, S. (eds) Natural Products From Marine Algae. Methods in Molecular Biology, vol 1308. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2684-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-2684-8_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2683-1

  • Online ISBN: 978-1-4939-2684-8

  • eBook Packages: Springer Protocols

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