Abstract
Callus and cell suspension cultures of licorice (Glycyrrhiza glabra) have been established in Gamborg's B5 medium supplemented with 2% sucrose, 1.0 mg 1-1 2,4-dichlorophenoxyacetic acid and 0.1 mg 1-1 kinetin. Biomass accumulation was affected by the density and age of inoculum, vessel size and sucrose level. 8% sucrose medium in 2-1 flasks induced biphasic growth, with fructose as the only source of carbon and energy in the second phase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- Y:
-
biomass yield
- P:
-
biomass productivity
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- td:
-
doubling time
References
Ayabe S, Iida K & Furuya T (1986) Induction of stress metabolites in immobilized Glycyrrhiza echinata cultured cells. Plant Cell Rep. 3: 186–189
Drapeau D, Blanch H W & Wilke C R (1986) Growth kinetics of Discorea deltoidea and Catharanthus roseus in batch culture. Biotechnol. Bioeng. 28: 1555–1563
Fowler M W (1982) Substrate utilization by plant cell cultures. J. Chem. Tech. Biotecnol. 132: 338–346
Gamborg O L, Miller L A & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root culture. Expl. Cell Res. 50: 151–158
Hayashi H, Fukui H & Tabata M (1988) Examination of triterpenoids produced by callus and cell suspension cultures of Glycyrrhiza glabra. Plant Cell Rep. 7: 508–511
Henry M, Edy A-M & Marty B (1984) Isolation of licorice protoplasts (Glycyrrhiza glabra L. var. typica Reg. et Hed.) from cell suspension cultures not producing glycyrrhetinic acid. C. R. Acad. Sc. Paris, 3: 899–903
Kobayashi M, Noguchi H & Sankawa V (1985) Formation of chalcones and isoflavones by callus culture of Glycyrrhiza uralensis with different production patterns. Chem Pharm. Bull. 33: 3811–3816
Mac Carthy J J, Ratcliffe D & Street H E (1980) The effect of nutrient medium composition on the growth cycle of Catharanthus roseus G. Done cells grown in batch culture. J. Exp. Bot. 31: 1315–1325
Merillon J M, Rideau M & Chenieux J C (1984) Influence of sucrose on levels of ajamlicine, serpentine and tryptamine in Catharanthus roseus cells in vitro. Planta Med. 48: 497–501
Morris P, Rudge R, Cresswell R & Fowler M W (1989) Regulation of product synthesis in cell cultures of Catharanthus roseus. V. Long-term maintenance of cells on a production medium. Plant Cell Tiss. Org. Cult. 18: 79–90
Rose D & Martin S M (1974) Parameters for growth measurements in suspension cultures of plant cells. Can. J. Bot. 52: 903–912
Stafford A, Smith L & Fowler M W (1985) Regulation and product synthesis in cell cultures of Catharanthus roseus (L) G. Don. Plant Cell Tiss. Org. Cult. 4: 83–94
Tamaki E, Morishita I, Nishida K, Kato K & Matsumoto T (1973) Process for preparing licorice extract-like material for tobacco flavoring. USA patent: 3710512
Widholm J M (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Tech. 47: 189–194
Yoo S H & Kim S S (1976) Studies in tissue culture of medicinal plants (II). Tissue culture of Glycyrrhiza glabra L. var. glandulifera Reg et Herder. Kor. J. Pharmacol. 7: 55–57
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Arias-Castro, C., Scragg, A.H., Stafford, A. et al. Growth characteristics of Glycyrrhiza glabra cell suspension cultures. Plant Cell Tiss Organ Cult 34, 77–82 (1993). https://doi.org/10.1007/BF00048466
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00048466