Skip to main content
SpringerLink
Log in

Tissue culture of Hypericum brasiliense Choisy: Shoot multiplication and callus induction

  • Orginal Research Papers
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Hypericum brasiliense, a non-domesticated plant has been shown to have useful medicinal properties. This plant has not been cultivated so a protocol for mass propagation based on selection of superior clones was developed and a protocol established for the culture of callus cells that could be used for in vitro metabolite production. A micropropagation method based on amplification of nodal buds was developed, by selection, from ten seedling clones that were examined for growth rate, multiplication rate and rooting. The effect of various basal media, growth regulator types and concentrations were examined for optimal callus induction. Optimal callus induction occured on either Murashige and Skoog's or Gamborg's media supplemented with 1 to 2 mg l−1 of 2,4-dichlorophenoxyacetic acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

B5:

Gamborg's medium

2,4-Dscd:

2,4-dichlorophenoxyacetic acid

IAA:

indolacetic acid

MS:

Murashige & Skoog's medium

NAA:

naphtaleneacetic acid

References

  • Bütter KB, Bütter B & Schaffner W (1994) In vitro propagation of Hypericum perforatum: impact of thidiazuron and 6-benzyloaminopurine. Abstracts of the VIII IAPTC Congress, Firenze, Italy (p. 62)

  • Cardona ML, Fernandez I, Pedro JR & Serrano (1990) Xanthones from Hypericum reflexum. Phytochemistry 29: 3003–3006

    Google Scholar 

  • Cellárová E, Kimáková K & Brutovská R (1992a) Multiple shoot formation and phenotypic changes of R0 regenerants in Hypericum perforatum L. Acta Biotechnol 12: 445–452

    Google Scholar 

  • Cellárová E, Kimáková K & Brutovská R (1992b) Multiple shoot formation in Hypericum perforatum L. and variability of R0. Biol Plant 34 (Suppl): 536

    Google Scholar 

  • Decosterd LA, Stoecki-Evans H, Chapuis JC, Sorda B & Hostettmann K (1989) New cell-growth inhibitory cyclohexadienone derivatives from Hypericum calycinum. Helv. Chim. Acta 72: 1833–1845

    Google Scholar 

  • Fehr MJ, Carpenter SL & Petrich JW (1994) The role of oxygen in the photoinduced antiviral activity of hypericin. Bioorgan. & Med. Chem. Let. 4: 1339–1344

    Google Scholar 

  • Fehr MJ, McCloskey MA & Petrich JW (1995) Light induced acidification by the antiviral agent hypericin. J. of the Am. Chem. Soc. 117: 1833–1836

    Google Scholar 

  • Fernandez ML & Alcaraz MJ (1991) Antiinflamatory activity and inhibition of arachidonic acid metabolism by flavoniods. Agents and Action 32: 283–288

    Google Scholar 

  • Gai F, Fehr MJ & Petrich JW (1993) Ultrafast excited-state processes in the antiviral agent hypericin. J. of the Am. Chem. Soc. 115: 3384–3385

    Google Scholar 

  • Gamborg OL, Miller PA & Ojima K (1986) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158

    Google Scholar 

  • Grattapaglia D & Machado MA (1990) Micropropagação. In: Torres AC & Caldas LS (eds) Técnicas e Aplicações da Cultura de Tecidos de Plantas (pp 99–170). ABCTP/EMBRAPA-CNPH, Brasilia

    Google Scholar 

  • Illg RD, (1990) Variação somaclonal. In: Torres AC & Caldas LS (eds) Técnicas e Aplicações da Cultura de Tecidos de Plantas (pp 287–297). ABCTP/EMBRAPA-CNPH, Brasília

    Google Scholar 

  • Ishiguro K, Yamaki M, Kashihara M, Takagi S & Isoi K (1990) A chromene from Hypericum japonicum. Phytochemistry 29: 1010–1011

    Google Scholar 

  • Jimenez CR (1980) Hipericáceas. In: Reitz R (ed) Flora Ilustrada Catarinesee (pp 1–33). CNPq/IBDF/HBR, Itajaí

    Google Scholar 

  • Kaneda N, Pezzuto JM, Soejarto DD, Kingharn AD, Farnsworth NR, Santisuk T, Tuchinda P, Udchachon J & Reutrakul V (1991) Plant anticancer agents 4D. New cytotoxic flavonoids from Mutingia calobura roots. J. Nat. Prod. 54: 196–206

    Google Scholar 

  • Kartnig T & Brantner A (1990) Secondary constituents in cell cultures of Hypericum perforatum and Hypericum maculatum. Planta Med. 56: 634

    Google Scholar 

  • Liebes L, Mazur Y, Freeman D, Lavie D, Lavie G, Kudler N, Mendoza S, Levin B, Hoechster H & Mervelo D (1991) A method for the quantification of hypericin, an antiviral agent, in biological fluids by High Performance Liquid Chromatography. Anal Biochem 195: 77–85

    Google Scholar 

  • Moraleda G, Wu TT, Jibert AR, Aldrich CE, Condreay LD, Larsen SH, Tang JC, Colarino JM & Masen WS (1993) Inhibition of duck hepatitis B virus replication by hypericin. Antiviral Res 20: 235–248

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  • Nakanishi S, Kobayashi E, Nakano H, Suzuki K & Tamaoki T (1989) Hypericin and pseudohypericin specifically inhibit protein kinase c: possible relation to their antiretroviral activity. Biochem Biophys Res Communications 165: 1207–1213

    Google Scholar 

  • Oganesyan ET, Saraf AF, Simonyan AV & Shiryaev IN (1991) Examining the structure activity relationship in the series of flavonoids 9. Allergenic activity of chalcones. Khimiko Farmatsevtichesskii Zhurnal 25: 18–21

    Google Scholar 

  • Picq M, Cheav SL & Pringent AF (1991) Effect of two flavonoids compounds on central nervous systems. Analgesic activity. Life Sci 49: 1979–1988

    Google Scholar 

  • Rocha LM (1991) Estudo quimico e farmacológico de Hypericum brasiliense Choisy. M.S. Dissertation NPPN/UFRJ, Rio de Janeiro

  • Saraf AS & Oganesyan ET (1991) Flavonoids as potential antiallergic agents-A review. Khimiko Farmatseutticheskii Zhurnal 25: 4–7

    Google Scholar 

  • Seabra RM & Correia Alves A (1989) Mangiferin and chlorogenic acid from Hypericum species. Planta Med 4: 404–405

    Google Scholar 

  • Seabra RM & Correia Alves A (1991) Quercetin-3′-sulphate from Hypericum elodes. Phytochemistry 30: 1344–1345

    Google Scholar 

  • Serkedjieva J, Manolokova N, Zgonia K, Nowosielska I, Zawilinska B & Grzybek J (1990) Sarothralin G. a new antimicrobial compound from Hypericum japonicum. Planta Med 56: 274–276

    Google Scholar 

  • Stevenson NR & Lenard J (1993) Antiretroviral activities of hypericin and rose bengal-Phodynamic effects on friend leukenia virus infection of mice. Antiviral Res 21: 119–128

    Google Scholar 

  • Weber ND, Muray BK, North JA & Wood SG (1994) The antiviral agent hypericin has in vitro activity against HSV-1 through non-specific association with viral and cellular membranes. Antiviral Chemistry & Chemotherapy 5: 83–90

    Google Scholar 

  • White PR (1943) A Handbook of Plant Tissue Culture. The Jackes Cattel Press, Lancaster

    Google Scholar 

  • Yazaki K & Okuda T (1990) Procyadins in callus and multiple shoot cuhures of Hypericum erectum. Planta Med 56: 490–495

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, Federal University of Rio de Janeiro, P.O. Box: 68011, 21944-970, Rio de Janeiro, Brasil

    M. A. Cardoso & D. E. de Oliveira

  2. Rio de Janeiro Botanical Garden, Rua Pacheco Leão 915, 22460-030, Rio de Janeiro, Brasil

    M. A. Cardoso

Authors
  1. M. A. Cardoso
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. E. de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cardoso, M.A., de Oliveira, D.E. Tissue culture of Hypericum brasiliense Choisy: Shoot multiplication and callus induction. Plant Cell Tiss Organ Cult 44, 91–94 (1996). https://doi.org/10.1007/BF00048184

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00048184

Key words

Search

Navigation