Abstract
A successful procedure has been designed for the regeneration of plantlets from leaf sections of the self-pollinating species,Medicago suffruticosa. Callus growth was promoted by a 4-week culture period on liquid Kao's medium containing 4.9 μM benzyladenine and 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-d), followed by a 4-day treatment in which the benzyladenine was elevated to 44.4 μM. Shoots/plantlets were observed after 3–4 weeks culture on growth regulator-free agar-solidified medium. Under these conditions, the regeneration frequency from callus was 18% and a histological study showed that this regeneration was through somatic embryogenesis. The growth regulator treatment, with a relatively high concentration of growth regulators (44.4 μM benzyladenine) for a short time period (4 days), is important for inhibiting polyphenol compounds and for stimulating callus growth and plant regeneration.
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Abbreviations
- 2,4-d :
-
2,4-dichiorophenoxyacetic acid
- BA:
-
benzyladenine
- NAA:
-
α-napthaleneacetic acid
References
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Li, XO., Demarly, Y. Somatic embryogenesis and plant regeneration inMedicago suffruticosa . Plant Cell Tiss Organ Cult 44, 79–81 (1996). https://doi.org/10.1007/BF00045916
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DOI: https://doi.org/10.1007/BF00045916