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In vitro culture and plant regeneration of large flowered purslane

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Abstract

Culture conditions were established for callus induction from a range of Portulaca grandiflora Hook tissues. Rapidly growing calli were obtained on Murashige and Skoog medium with stem-, leaf- and sepal-derived explants. Plant regeneration via organogenesis was explant-origin dependent with hypocotyl tissues giving the highest shooting frequency. Light conditions, pH and carbon source had a pronounced effect on the percentage of explants regenerating buds and the number of buds formed. It was possible to establish stable regenerated plants in the glasshouse.

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Abbreviations

BA:

6-benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthaleneacetic acid

IAA:

indoleacetic acid

MS:

Murashige and Skoog (1962) medium

References

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Author notes

  1. Badr-Din Rossi-Hassani

    Present address: Laboratoire de Biochimie & Biologie Moléculaire, Faculté des Sciences-Dhar Mehraz, Fès B.P. 1796, Morocco

Authors and Affiliations

  1. Laboratoire de Phytogénétique Cellulaire, Université de Lausanne, 1015, Lausanne, Switzerland

    Badr-Din Rossi-Hassani & Jean-Pierre Zryd

Authors
  1. Badr-Din Rossi-Hassani
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  2. Jean-Pierre Zryd
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Rossi-Hassani, BD., Zryd, JP. In vitro culture and plant regeneration of large flowered purslane. Plant Cell Tiss Organ Cult 41, 281–283 (1995). https://doi.org/10.1007/BF00045093

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  • DOI: https://doi.org/10.1007/BF00045093

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