Abstract
Studies on aquatic ecosystems at the trophic level of bacteria include population succession and the movement of species through the water column. Factor and path analysis of environmental parameters and the bacterial profiles indicate that the bacterial populations are under the control of environmental factors. The most important environmental factors in the Canadian study reported here are temperature followed by oxygen levels, nutrient levels, and ion concentrations. A major revolution in investigative approaches has begun in aquatic bacterial population studies using technology based on molecular methods. Finger print analysis of bacterial 16S RNA (molecular phylogeny) has not only changed the classification of bacteria but also the approach to solving environmental problems. The bacterial groups have been placed into species that are more functionally and ecologically aligned. Uncultured mixed biomass can be examined by gene probes for both procaryotes and eucaryotes to identify specific nucleotide sequences. Aquatic ecosystem health is maintained by the balanced biota and the process of biodegradation is an important stage in bioremediation. Control of toxic wasters in the waters and groundwaters can be accomplished byin situ bioremediation using indigenous microorganisms as demonstrated by the field study reported by Litchfieldet al. (1990).
Similar content being viewed by others
References
Achtman, M. & G., Pluschke, 1986. Clonal analysis of descent and virulence among selectedE. coli. Ann. Rev. Microbiol. 40: 185–210.
Bell, C. R., M. A. Holder-Franklin & M. Franklin, 1981. Diurnal variations of the heterotrophic bacteria in the sediment and water of a small woodland stream. In: D. B. Carlisle (ed.), Methods of studying population shifts in aquatic bacteria in response to environmental change. Inland Waters Direct. Sci. Ser. 124: 77–89.
Bell, C. R., M. A., Holder-Franklin & M., Franklin, 1982. Correlations between predominant heterotrophic bacteria and physico-chemical water quality parameters in two Canadian rivers. Appl. Environ. Microbiol. 43: 269–283.
Cashion, P., M. A., Holder-Franklin, J., McCully & M., Franklin, 1977. A rapid method for the base ratio determination of bacterial DNA. Analyt. Biochem. 81: 461–466.
Holder-Franklin, M. A., 1981. Methods of studying population shifts in aquatic bacteria in response to environmental change. In: D. B. Carlisle (ed.), Methods of studying population shifts in aquatic bacteria in response to environmental change. Inland Waters Direct. Sci. Ser., 124.
Holder-Franklin, M. A., 1984. Mathematical analysis of bacterial test responses. In: Bacteriologie Marine. Editions du C. N. R. S. Paris, 331: 71–77.
Holder-Franklin, M. A. & L. J., Wuest, 1983. Population dynamics of aquatic bacteria in relation to environmental change as measured by factor analysis. J. Microbiol. Meth. 1: 209–227.
Holder-Franklin, M. A., M., Franklin, P., Cashion, C., Cormier & L., Wuest, 1978. Population shifts in heterotrophic bacteria in a tributary of the St. John River as measured by taxometrics. In: M. W., Loutit & J. A. R., Miles (eds),Microbial Ecology, pp. 44–50, Springer Verlag, Berlin, Heidelberg, New York.
Holder-Franklin, M. A., A., Thorpe & C. J., Cormier, 1981. Comparison of numerical taxonomy and DNA-DNA hybridization in diurnal studies of river bacteria. Can. J. Microbiol. 27: 165–184.
Litchfield, C. D., C. C., Chein, J. R., Buttram, S. H., Shoemaker, R. M., Seibert & C. H., Ward, 1990. Field demonstration of the biodegradation of benzene and chlorinated aliphatics at an industrial site. Ann. Meet. Amer. Soc. Microbiol. 17: 17.
King, W., S. M. Raposa, J. E. Warshaw, A. R. Johnson, D. Lane, J. D. Klinger & D. N. Halbert, 1990. A colorimetric assay for the detection of Listeria using nucleic acid probes. In: A. J. Miller, J. L. Smith & G. A. Somkuti (eds), Foodborne Listeriosis. pp. 117–124. Soc. Indust. Microbiol.
Knight, I. T., S., Shultz, C. W., Kaspar & R. P., Colwell, 1990. Direct detection ofSalmonella spp. in estuaries by using a DNA probe. Appl. Environ. Microbiol. 56: 1059–1066.
Mathan, K. K., P. P., Ramaswami, C., Suyambulingam & W., Jebarani, 1977. Identification of factors influencing microbial populations in red and black soils by path analysis. Madras Agric. J. 64: 549–553.
Olsen, G. J., D. J., Lane, S. J., Giovannoni, N. R., Pace & D. A., Stahl, 1986. Microbial ecology and evolution: a ribosomal approach. Ann. Rev. Microbiol. 40: 337–365.
Parsons, G., 1988. Development of DNA probe-based commercial assays. J. Clin. Immunoassay 11: 152–159.
Wright, S., 1934. The method of path coefficients. Ann. Math. Statist. 5: 161–215.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Holder-Franklin, M.A. Aquatic microorganisms: processes, populations, and molecular solutions to environmental problems. J Aquat Ecosyst Stress Recov 1, 253–262 (1992). https://doi.org/10.1007/BF00044167
Issue Date:
DOI: https://doi.org/10.1007/BF00044167