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Experimental control of stress hormone levels in fishes: techniques and applications

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Summary

We have attempted to critically review the methods used to experimentally controlin vivo levels of circulating stress hormones (cortisol, epinephrine, norepinephrine) in fish, and to suggest which of them are most suitable for future studies (Table 4). From the discussion it is evident that there are numerous choices for achieving elevated hormone concentrations. However, the method chosen should match the temporal pattern and the degree of hormone elevation that is concordant with the type of stress one is trying to simulate; in other words be physiological. With regard to inhibition of hormone release as a tool forin vivo physiological investigations, it appears that few of these methods are suitable for widespread usage. However, surgical denervation of the chromaffin tissue, and the inhibition of cortisol synthesis by implantation of cortisol-laden pellets within the forebrain nuceli or by the administration of metopirone, hold the most promise for eliminating the release of catecholamines and cortisol respectively.

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  1. M. M. Vijayan

    Present address: EVS Environmental Consultants, 195 Pemberton Ave., V7P 2R4, North Vancouver, B.C., Canada

Authors and Affiliations

  1. Department of Biology, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada

    A. K. Gamperl

  2. Department of Biology, University of Ottawa, K1N 6N5, Ottawa, Ontario, Canada

    M. M. Vijayan

  3. Department of Zoology, University of Cambridge, Downing Street, CB2 3EJ, Cambridge, United Kingdom

    R. G. Boutilier

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Gamperl, A.K., Vijayan, M.M. & Boutilier, R.G. Experimental control of stress hormone levels in fishes: techniques and applications. Rev Fish Biol Fisheries 4, 215–255 (1994). https://doi.org/10.1007/BF00044129

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