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Induction of microspore embryogenesis in Camellia japonica cv. Elegans

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Abstract

Three methods of microspore culture were tested for the induction of microspore embryogenesis in Camellia japonica L. cv. Elegans. Culture was performed on 17 different media consisting of Murashige and Skoog (MS) and N6 basal media with different combinations of carbon, growth regulators, serine and glutamine. Microspore suspensions plated over solid MS medium containing 4.5 μM 2,4-dichlorophenoxyacetic acid and 0.5 μM kinetin, with sucrose (MS6) or glucose (MS9) were seen as the best culture conditions for induction of embryogenesis. The development of microspore derived proembryos was obtained in MS medium supplemented with 2.2 μM N6-benzyladenine (MS10) and reached the highest level when the microspores were cultured in MS6 inducing medium. The development of microspore-derived embryos ceased at the maturation stage.

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Abbreviations

BA:

N6-benzyladenine

2,4-d :

2,4-dichlorophenoxyacetic acid

References

  • Bajaj, YPS (1990) Haploids in Crop Improvement I. Biotechnology in Agriculture and Forestry, Vol 12 (pp 101–541). Springer-Verlag, Berlin, Heidelberg

    Google Scholar 

  • Chen, Z (1990) Rubber (Hevea brasiliensis Muell. Arg.): In vitro production of haploids. In: Baja, YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 12 (pp 215–236). Springer-Verlag, Berlin, Heidelberg

    Google Scholar 

  • Chen, Z, Chen, F, Chien, C, Wang, C-h, Chang, S-c, Hsu, H-c, Ou, H-h, Ho, Y-t & Lu, T-m (1978) A process of obtaining pollen plants of Hevea brasiliensis Muell.-Arg. Sci. Sin. 22: 81–90

    Google Scholar 

  • Chu, CC (1978) The N6 medium and its applications to anther culture of cereal crops. Proc. Symp. Plant Tissue Culture Beijing (pp 43–50). Science Press, Peking

    Google Scholar 

  • Lianfang, F (1990) Litchi (Litchi chinensis Sonn.): In vitro production of haploids. In: Bajaj, YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 12 (pp 265–274). Springer-Verlag, Berlin, Heidelberg

    Google Scholar 

  • McClintock, B (1929) A method for making aceto-carmin smears permanent. Stain Technol. 4: 53–56

    Google Scholar 

  • Milewska-Pawliczuk, E (1990) Apple (Malus domestica Borkh.): In vitro induction of androgenesis. In: Bajaj, YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 12 (pp 250–265). Springer-Verlag, Berlin, Heidelberg

    Google Scholar 

  • Milewska-Pawliczuk, E & Kubicki, B (1977) Induction of androgenesis in vitro in Malus domestica. Acta Hort. 78: 271–276

    Google Scholar 

  • Murashige, T & Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Pedroso, MC & Pais, MS (1992) A SEM and X-ray microanalysis study during induction of morphogenesis in Camellia japonica L. Plant Sci. 87: 99–108

    Google Scholar 

  • Pedroso, MC & Pais, MS (1993) Direct embryo formation in leaves of Camellia japonica L. Plant Cell Rep. 12: 639–643

    Google Scholar 

  • Pedroso-Ubach MC (1991) Contribuição para a preservção e o melhoramento de Camellia japonica L. Faculdade de Ciências da Universidade de Lisboa. Master Thesis (English Abstract) (pp 23–50;89–103)

  • Raghavan, V & Nagmani, R (1989) Cytokinin effects on pollen embryogenesis in cultured anthers of Hyoscyamus niger. Can. J. Bot. 67: 247–257

    Google Scholar 

  • Sunderland, N & Dunwell, JM (1974) Pathways in pollen embryogenesis. In: Street, HE (Ed) Tissue Culture and Plant Science (pp 141–167). Academic Press, New York

    Google Scholar 

  • Taylor, DC, Weber, N, Underhill, EW, Pomery, MK, Keller, WA, Scowcroft, WR, Wilen, RW, Moloney, MM & Molbrook, LA (1990) Storage-protein regulation and lipid accumulation in microspore embryos of Brassica napus L. Planta 181: 18–26

    Google Scholar 

  • Wang, C, Chu, Z & Sun, C (1975) The induction of Populus pollen plants. Acta Bot. Sin. 17: 56–59

    Google Scholar 

  • Widholm, JM (1972) The use of fluoresceine diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47: 189–194

    Google Scholar 

  • Zhang YX, Lespinasse Y & Chevreau E (1990) In vitro androgenesis in apple (Malus domestica Borkh.). Abstracts WIIth International Congress on Plant Tissue and Cell Culture. Amsterdam, June 24–29, A6-10, 185

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    1. M. Cristina Pedroso
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    2. M. Salomé Pais
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    Pedroso, M.C., Pais, M.S. Induction of microspore embryogenesis in Camellia japonica cv. Elegans. Plant Cell Tiss Organ Cult 37, 129–136 (1994). https://doi.org/10.1007/BF00043606

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    • DOI: https://doi.org/10.1007/BF00043606

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