Abstract
Four tetraploid potato genotypes (194.10, 199.13, 201.5, 201.12) were examined in anther culture. The androgenic responses were in general high. Cv. 199.13 contributed with the best response, varying between 0.38 and 0.55 embryoids per anther. Gellan gum or potato starch were used as gelling agents in a double-layer medium. Anthers incubated on potato starch gave a higher embryo yield in the beginning of the culture period, compared to anthers cultivated on gellan gum. The number of embryoids per anther, however, was higher on gellan gum at the end of the culture period. Anther cultures of potato were incubated in two different temperatures (20 °C and 25 °C), and the highest embryo yield was obtained in 25 °C except for genotype 201.12 where no difference was found between the two temperatures. Experiments with pollen germination in various temperatures (10 °C and 20 °C) were correlated to anther culture experiments. Also in case of pollen germination, genotype 201.12 was temperature-independent, while germination was stimulated by higher temperatures in the other genotypes.
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Calleberg, E.K., Kristjansdottir, I.S. & Johansson, L.B. Anther cultures of tetraploid Solanum genotypes—the influence of gelling agents and correlations between incubation temperature and pollen germination temperature. Plant Cell Tiss Organ Cult 19, 189–197 (1989). https://doi.org/10.1007/BF00043346
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DOI: https://doi.org/10.1007/BF00043346